米非司酮对垂体移植诱发小鼠子宫腺肌症的影响  

Treatment of experimentally induced uterine adenomyosis with mifepristone (RU486)

作　　者：周应芳[1] 守隆夫[2] 郑淑蓉 

机构地区：[1]北京大学第一医学院妇产科北京100034 [2]日本东京大学理学部生物科学研究科内分泌研究室

出　　处：《北京医科大学学报》2000年第6期500-502,507,共4页Journal of Peking University(Health Sciences)

摘　　要：目的 :了解米非司酮 (RU486 )治疗对SHN系小鼠子宫腺肌症的影响。方法 :2 0只 7周龄未受过孕的雌性小鼠开腹行脑垂体子宫内移植手术 ,用来促使子宫腺肌症的发病。术后 17周 ,将 2 0只小鼠随机分为两组。治疗组投予含RU486 (2 0mg·kg-1)的饲料 ,对照组给予不含RU486的相同饲料。 4周后处死小鼠 ,取出子宫和卵巢称重 ,计数子宫表面突出的腺肌症结节。结果 :治疗组和对照组相比 ,子宫较细、颜色较浅。子宫湿重治疗组明显低于对照组。治疗组和对照组各有 8只小鼠子宫表面可见突出的腺肌症结节 ,结节发生率两组比较 ,差异无显著性 ,但治疗组的结节数量明显少于对照组。显微镜检查证实 ,子宫表面的突出结节均为子宫腺肌症。治疗组子宫内膜间质细胞致密化 ,腺肌症组织的腺体分泌明显受到抑制。腺肌症发生率两组相同 (8/ 10比 8/ 10 )。结论 :RU486治疗 4周虽不能治愈小鼠子宫腺肌症 ,但可以缩小子宫和腺肌症病灶 。Objective: To investigate the effectiveness of Mifepristone (RU486) on experimentally induced uterine adenomyosis in SHN mice. Methods: A single pituitary was transplanted in the lumen of the right uterus of 20 virgin female SHN mice at the age of 7 weeks respectively , as pituitary grafting quickly induced the development of uterine adenomyosis. Mice were randomly divided into 2 groups, 10 of each, 17 weeks after pituitary grafting. The treatment group received 4 weeks of RU486 treatment. All mice were then killed and the uteri and ovaries were weighed. The right uteri were examined under a 20 fold microscope and counted for the extrauterine nodules, which was the characteristic of advanced adenomyosis. Results: Uteri in treatment group were thinner and less congestive compared with those of the control group. Compared with the controls, RU486 treatment significantly reduced the uterine wet weight. The number of extrauterine nodules detected in each of the 8 mice in either group was significantly lower in RU486 treated group than that of the controls, although the incidence was similar between the two groups. Microscopic examination confirmed that all uteri showing extrauterine nodules were with advanced adenomyosis. In RU486 treated mice, the endometrial stromal cells became dense and the secretion activity of ectopic endometrial glands was markedly suppressed compared with the control group. The incidence of uterine adenomyosis was similar between RU486 treated group (8/10 mice, 80%) and the control group (8/10 mice, 80%). Conclusion: Four weeks of RU486 treatment shrunk the uterus and suppressed its adenomyosis in a mouse model although it could not cure the disease.

关 键 词：药物疗法 子宫腺肌症 米非司酮 药理学 垂体移植 

分 类 号：R711.710.5[医药卫生—妇产科学]