TGF-β1、IL-10在门静脉预输注供者凋亡脾脏细胞大鼠移植心脏模型中的表达  

Expression of IL-10 and TGF-β1 in a rat cardiac model established by administration of donor apoptotic spleen cells via portal vein before transplantation

作　　者：章佰承[1] 叶明[1] 安守宽[1] 乔友进[1] 李君权[1] 夏求明[1] 

机构地区：[1]哈尔滨医科大学附属第二医院心外科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2013年第3期224-228,共5页Journal of Harbin Medical University

摘　　要：目的输注供者凋亡细胞建立大鼠同种异体心脏移植模型,探讨凋亡细胞诱导免疫耐受的作用机制。方法实验动物分为3组:A组为对照组;B组为实验组,心脏移植前经门静脉输注供者来源的凋亡脾脏细胞;C组为免疫抑制剂组,移植前后给予CsA。观察各组移植心脏存活时间,组织病理学改变,血清IL-2、IL-10及TGF-β1含量,并通过单向混合淋巴细胞培养判定耐受是否具有抗原特异性。结果 B组移植心脏存活时间较A组显著延长,排斥反应程度减轻,受者对供者产生抗原特异性耐受;C组移植心脏存活时间最长,但其免疫抑制作用缺乏抗原特异性。在心脏移植术后B组血清中IL-2水平较对照组显著降低,而IL-10及TGF-β1显著升高。结论通过预输注供者凋亡细胞的方法可以诱导大鼠同种异体心脏移植的免疫耐受,并且此种耐受具有抗原特异性。IL-10及TGF-β1在此过程中可能起着重要作用。Objective To study the mechanism of immune tolerance induced by administration of donor apoptotic cells into a rat cardiac allogenic model.Methods The animals were divided into 4 groups at random：Group A was control group; Group B was experimental group,administration of donor apoptotic cells via portal vein before allogenic heart transplantation; In group C,the animals were injected intraperitoneally with CsA before transplantation till post transplantation.The survival time of donor heart and histological changes were observed.Serum concentrations of IL-2,IL-10 and TGF-β1 of recipients and mixed lymphocyte reaction（MLR）were measured to determine whether the immune tolerance was antigenic specificity.Results The survival time of heart allografts in the group B was significantly longer than that in group A,and rejection level was lower and receptor showed an antigen specific character.In group B the serum concentration of IL-2 was significantly lower than that in group A,but the serum concentrations of IL-10 and TGF-β1 were higher than those in group A.Conclusion Administration of donor apoptotic cells via portal vein before allogenic heart transplantation can induce immune tolerance,which shows an antigen specific character.IL-10 and TGF-β1 may play important roles in this process.

关 键 词：凋亡细胞 免疫耐受 心脏移植 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]