白藜芦醇对过氧化氢诱导鼠晶状体上皮细胞凋亡保护作用的实验研究  

Protective effect of resveratrol on human lens epithelial cells against H_2O_2-induced apoptosis

作　　者：陈雪芳[1] 刘忠鑫[1] 陈炳荣[1] 白洁[2] 郑轶[2] 

机构地区：[1]海南省三亚市人民医院眼科,海南三亚572000 [2]哈尔滨医科大学附属第一医院眼科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2013年第3期229-231,F0004,共4页Journal of Harbin Medical University

基　　金：黑龙江省青年科学基金资助项目(QC2011C131)

摘　　要：目的探讨白藜芦醇对过氧化氢(H2O2)诱导鼠晶状体上皮细胞凋亡损伤的保护作用。方法取健康SD大鼠透明晶状体60只,随机分为5组,每组12只,分别给予高、中、低剂量(5、10、20μmol/L)的白藜芦醇和100μmol/L H2O2,利用透射电镜观察超微结构变化并测定凋亡因子caspase-3及caspase-9的表达。结果伴随白藜芦醇给药浓度的增加,透射电镜下鼠晶状体上皮细胞损伤的程度减轻,此外,白藜芦醇对caspase-3及caspase-9的表达具有抑制作用。结论白藜芦醇可以抑制H2O2诱导的晶状体上皮细胞的凋亡,对氧化损伤性白内障具有预防作用,为寻求有效防治白内障药物提供了可靠的实验依据。Objective To discuss the protective effect of resveratrol on human lens epithelial cells （HLEC） against H2O2-induced apoptosis.Methnds Sixty lenses of healthy SpragueDawley （SD） rats were randomly devided into 5 groups,then incubated with different concentration of resveratrol （5,10,20 μmol/L） and H2O2,ultrastructure changes were observed,and apoptosis expression of caspase-3 and caspase-9 were assayed under transmission electron microscope.Results The damage of human lens epithelial cells were alleviated with resveratrol concentration elevated,and the expression of caspase-3 and caspase-9 was also inhibited.Conclusion Resveratrol may inhibit H2O2-induced apoptosis of HLEC,and has protective function against oxidative damage cataract.It provides reliable evidence for pursuing effective medicine to prevent and treat cataract.

关 键 词：白藜芦醇 人晶状体上皮细胞 CASPASE-3 CASPASE-9 凋亡 

分 类 号：R988.1[医药卫生—药品] R776.1[医药卫生—药学]