基于mtCOⅠ基因的三种稻飞虱DNA条形码构建及应用  

DNA barcoding application to three species of rice planthoppers based on mtCOⅠ gene

作　　者：谢家楠[1] 郭建军[1] 汪学俭[1] 金道超[1] 

机构地区：[1]贵州大学昆虫研究所,贵州山地农业病虫害重点实验室,贵阳550025

出　　处：《植物保护学报》2013年第6期540-544,共5页Journal of Plant Protection

基　　金：贵州省科技攻关项目(黔科合NY字[2010]3064号);贵州省科技厅自然科学研究项目(黔教2010011);国家"973"计划前期研究专项(2009CB125908)

摘　　要：为构建白背飞虱、褐飞虱、灰飞虱3种稻飞虱DNA条形码，准确鉴定各虫态稻飞虱种类，随机选取35个供试虫体提取全基因组DNA，PCR扩增其中线粒体细胞色素氧化酶Ⅰ（mtCOⅠ）基因，分析所获得的基因组成特点，计算P距离值，构建系统发育树，并对稻飞虱若虫、卵进行种类鉴定。PCR扩增获得了长度为706 bp的稻飞虱mtCOⅠ基因片段，碱基组成特点种内、种间不同；3种稻飞虱的种间P距离值为25.53%~34.21%，种内为0~0.51%，种间值明显高于种内；系统发育树显示同种个体聚集在同一分支；同种卵、若虫的mtCOⅠ基因与已获得的成虫基因序列同源性最高值为100%，P距离值为0。研究表明，3种稻飞虱的DNA条形码可基于mtCOⅠ基因构建，且可准确鉴定各虫态。To construct the DNA barcoding of three species of main rice pest planthoppers, Nilaparvata lugens （St？l）, Sogatella furcifera （Horváth） and Laodelphax striatellus （Fallén） and identify them correctly at different stages, 35 specimens were selected randomly and their DNAs were extracted, from which mtCOⅠ gene was amplified by PCR, gene composition characters analyzed, P-distances calculated and phylogenetic tree constructed. At the same time, eggs and nymphs of these three species were identified using mtCOⅠ gene. The results showed that mtCOⅠ gene fragment obtained was 706 bp in length. The composition of base was different in intra-and inter-species. P-distances of inter-species were 25.53%-34.21% in three of those rice planthoppers, respectively, with 0-0.51% of intra-species. Phylogenetic analysis showed that the specimens of the same species were clustered in a single clade. The mtCOⅠ gene homology between eggs, nymphs of same species and obtained adults was as high as 100%, and P-distance was 0. The results indicated that the method of DNA barcoding based on the mtCOⅠ gene should be recommended for identification of the rice planthoppers at different stages.

关 键 词：稻飞虱 DNA条形码 卵和若虫 鉴定 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]