反相高效液相法测定石芽茶中山茶苷A的含量  

Determination of Camelliagenin A in Adinandra nitida by RP- HPLC

作　　者：刘元[1] 宋志钊[1] 刘布鸣[1] 

机构地区：[1]广西中医药研究院,广西南宁530022

出　　处：《中国民族民间医药》2013年第24期5-6,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西企业科技特派员专项项目(桂科产09321001);广西卫生厅重点科研课题(2011050);<广西壮族自治区壮药质量标准(第二卷)>标准研究项目(MZY2010044)

摘　　要：目的：建立石芽茶中山茶苷A的含量测定方法.方法：采用反相高效液相法,以XB-C18柱（4.6 mm×250mm,5μm）为分离柱,以乙腈-0.1%磷酸（20：80）为流动相,检测波长为330nm,柱温为室温.结果：山茶苷A在（20.2～323.2）μg/ml范围内,峰面积与其浓度呈良好线性关系（r=1.000）,平均回收率为98.8%,RSD为1.62%（n=6）.结论：该方法简便、准确、重复性好,可作为该药材含量测定方法.Objeetive To set up determination the contents of Camelliagenin A in Adinandra nitida. Methods RP - HPLC method was used, Chromatographic condition included XB- C18 （4. 6mm × 250mm, 5 （m） and the mobile phase consisting of acetonitrile -0. 1% phosphoric acid （20： 80） , with a detection wavelength of 330nm and the temperature to room temperature. Result The cal- ibration curves were linear in the range of 20. 2 - 323.2μg,/ml for Camelliagenin A, r = 1. 000, average recovery of Camelliagenin A was 98. 8%, RSD was 1.62% （n =6）. Conclusions The method is simple, accurate and repeatable, which applied in determina- tion of Camelliagenin A in Adinandra nitida.

关 键 词：石芽茶 反相高效液相法 山茶苷A 

分 类 号：R284.2[医药卫生—中药学]