BCBL-1细胞重组表达HIV-1 Nef蛋白对重组IL-6启动子的影响  

Effects of recombinant expression of HIV-1 Nef protein on recombinant interleukin 6 promoter in BCBL-1 cells

作　　者：赵丽娟[1] 韦红玉[1] 唐华英[1] 曾怡[1] 

机构地区：[1]右江民族医学院微生物学与免疫学教研室,广西百色533000

出　　处：《右江民族医学院学报》2013年第6期752-754,共3页Journal of Youjiang Medical University for Nationalities

基　　金：2010年国家自然科学基金(81071345)

摘　　要：目的探讨重组真核表达的HIV-1Nef蛋白对IL-6启动子活性的调节作用。方法将pNefF或pCI-neo与重组IL-6启动子虫荧光素酶报告质粒pIL6-Luc共转染BCBL-1细胞,同时用TPA刺激同样转染的BCBL-1细胞,于转染后24h、48h、72h、96h收集细胞,用Bright-Glo虫荧光素酶检测系统进行虫荧光素酶活性检测。结果未经TPA刺激的BCBL-1细胞中,转染96h时pNefF和pIL6-Luc共转染细胞中虫荧光素酶活性比pCI-neo和pIL6-Luc共转染细胞增加了3.66倍。BCBL-1细胞经TPA刺激后,转染96h时,pNefF和pIL6-Luc共转染细胞中虫荧光素酶下降1.72倍(P<0.05)。结论 Nef蛋白可在BCBL-1细胞内影响IL-6启动子活性。Abstract ： munodeficiency Objective virus type To investigate the role of recombinant eukaryotic expression of Nef of human im 1 （HIV--1） in activating interleukin 6 promoter. Methods Nef recombinant plas mid （pNefF） or control vector pCI--neo and luciferase reporter recombinant of IL--6 promoter （pIL6--Luc） were co--transfected to BCBL--1 cells. At the same time, other groups of BCBL--1 cell transfected with plas- mids as mentioned before were stimulated by 12--O-- tetradecanoylphorboI-- 13--acetate （TPA）. Cells were collected at 24 h, 48 h, 72 h and 96 h after transfection, respectively. Consequently, the promoter--driven lu- ciferase expression in BCBI.--1 cells was detected by Bright--Glo luciferase detect system. Results The promoter--driven luciferase expression increased by 3.66 times at 96 h after BCBL--lcells cotransfected with pNefF and pIL6--Luc without TPA when compared with ceils cotransfected with pCI--neo and pIL6--Luc. The promoter--driven luciferase expression decreased by 1.72 times at 96 h after BCBL--lcells co--transfected with pNefF and pIL6--Luc with TPA when compared with cells cotransfected with pCI--neo and plL6--Luc （P 〈 0.05） . Conclusion Expression of Nef in BCBL--1 cells contributed to activate IL--6 promoter--driv- en luciferase expression.

关 键 词：HIV-1 NEF BCBL--1细胞 白细胞介素6 

分 类 号：R373[医药卫生—病原生物学]