加工番茄马铃薯Y病毒一步法RT-PCR检测及CP基因的克隆与序列分析  

One-step RT-PCR Detection of Potato Virus Y Infecting Processing Tomato as Well as CP Gene Cloning and Sequence Analysis

作　　者：梁学超[1] 向本春[1] 程朝玲[1] 苏海娣[1] 郑银英[1] 

机构地区：[1]石河子大学生命科学学院,石河子大学农业生物技术重点实验室,新疆石河子832003

出　　处：《西南农业学报》2013年第6期2340-2344,共5页Southwest China Journal of Agricultural Sciences

基　　金：国际科技合作与交流专项(20072072);国家自然科学基金(31260420)

摘　　要：本文通过一步法RT-PCR技术,检测新疆加工番茄马铃薯Y病毒(Potato virus Y,PVY)感染率。结果表明,新疆加工番茄马铃薯Y病毒感染率为25%。选取10个样品利用RT-PCR技术,克隆CP基因,获得807 bp全长CP基因,编码268个氨基酸。CP基因序列比对分析与系统进化树分析表明,侵染新疆加工番茄的马铃薯Y病毒属于PVYN:O株系。The study analyzes potato virus Y prevalence of infection in processing tomato in Xinjiang by the one-step reverse transcription-pol- ymerase chain reaction（ RT-PCR）, the result showed that 25 % processing tomato could be infected with PVY. The CP gene included 807bp, and could encode a protein with 268 amino acids. CP gene of PVY strain were analyzed with informatics tools, and the molecular variation characters among different PVY strains were proved up. According to these results, PVY which infected processing tomato in Xinjiang was identified as an isolate of PVY^N：O strain.

关 键 词：马铃薯Y病毒 加工番茄 一步法RT—PCR CP基因 

分 类 号：S432.41[农业科学—植物病理学]