金线莲的组织培养与快速繁殖研究  

Study on Tissue Culture and Rapid Propagation of Anoectochilus roxburghii

作　　者：杨红丽[1] 胡靖锋[1] 徐学忠[1] 和江明[1] 宋爽[2] 

机构地区：[1]云南省农业科学院园艺作物研究所,云南昆明650205 [2]云南省农业科学院,云南昆明650205

出　　处：《西南农业学报》2013年第6期2485-2488,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技厅重点新产品开发计划(2012BB017);农业部公益性行业专项(201003029);云南省农业厅蔬菜新品种协作项目[云财农(2012)58号];国家大宗蔬菜产业技术体系(CARS-25-G-45)

摘　　要：以金线莲茎段为外植体,研究不同的消毒方法和激素水平对组织培养的影响,筛选出合适的培养基配方,并进行了移栽试验。结果表明,消毒以70%酒精处理30S+0.1%次氯酸钠处理20 min为宜;丛芽诱导的适宜培养基为MS+6-BA 0.8 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L为最佳;金线莲茎段中部为最佳外植体;生根培养基以1/2MS+NAA 1.0 mg/L+IBA 1.0 mg/L+去皮香蕉50 g+活性炭5 g为最佳。Stalks of Anoectochilus mxburghii were used as explants to study different disinfection methods and hormone levels influence on the tissue culture, and screen out appropriate culture medium and then do transplanting. It turned out that disinfection of 70 % alcohol need 30s, sterilization time of 0.1% sodium hypochlorite is 25 min, the most appropriate conditions is MS ＋ 6-BA 0. 8 mg/L ＋ NAA 0.5 mg/L ＋ KT 1.0 mg/L, the culture medium is suitable for the induction of cluster bud , stalks of Anoectochilus roxburghii is the most suitable explant. When the culture medium is 1/2MS ＋ NAA 1.0 mg/L ＋ IBA 1.0 mg/L ＋ peeled bananas 50 g ＋ charcoal active power 5 g, it was conducive to induction of roots.

关 键 词：金线莲 组织培养 快速繁殖 

分 类 号：S567.9[农业科学—中草药栽培]