1株产蛋高峰鸡H9N2的分离鉴定及HA基因序列分析  

Isolation,Identification and Molecular Characterization of H9N2 Isolate from Hen Flock at Peak of Laying

作　　者：张玉霞[1] 袁小远[1] 徐怀英[1] 亓丽红[1] 翟荣玲 王友令[1] 艾武[1] 

机构地区：[1]山东省农科院家禽研究所,山东济南250100 [2]山东莱芜市畜牧局,山东莱芜271100

出　　处：《西南农业学报》2013年第6期2605-2609,共5页Southwest China Journal of Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2010CQ044);山东省科技发展计划项目(2009GG10009006)

摘　　要：从产蛋率下降的蛋鸡群中分离到1株具有血凝性的病毒，经HI交叉抑制试验证实为H9N2病毒，命名为A／Chieken／Shandong／JN／11，病毒对SPF鸡基本无致病性，IVPI为0。对其HA基因进行克隆测序，HA基因裂解位点的序列为RSSRlGL，与弱毒基序一致；有7个潜在糖基化位点；受体结合位点除198位有变异外，其余均较保守；A／Chicken／Shandong／JN／11分离株分Gen-Bank上发表的近几年来山东参考株HA基因的核苷酸之间的同源性为89．9％-97．4％，氨基酸序列之间的同源性为93．4％～97．5％。系统进化树分析表明，该毒株与近年来国内流行的A／Chicken／zhejiang／ZLB／11禽流感毒株类似。A newly HgN2 isolate proved by cross HI test named A/Chieken/Shandong/JN/llwas collected from a hen fleck at the peak of laying. The biological test showed that the virus exhibited the IVPI value with 0, and the isolate share a lentogenic motif RSSR ↓ GL at the HA protein cleavage site, which indicate that the isolated virus was lcntogenic; Seven potential glycosylation sites were found and the receptor binding sites were conservative except the 198 site. The homology of HA nucleotide and amino acid sequences was 89.9 % - 97.4 % and 93.4 % - 97.5 % between the A/Chicken/Shandong/JN/11 and other reference strains in C, enBank from Shandong in recent years. Phylogenetic analysis showed that the A/Chicken/Shandong/JN/11 was similar to A/Chicken/zhejiang/ZLB/11.

关 键 词：H9N2病毒 HA基因 序列分析 

分 类 号：S831[农业科学—畜牧学]