家蚕黄茧限性品种不同性别LongSAGE文库的构建与分析  

SAGE Construction and Analysis between Males and Females of Yellow Cocoon sex-limited Strain of Silkworm,Bombyx mori

作　　者：甘丽萍[1,2] 刘仁华[1] 牛艳山[2] 席建[2] 司马杨虎[2] 徐世清[2] 

机构地区：[1]重庆三峡学院生命科学与工程学院,重庆万州404100 [2]苏州大学国家丝绸工程实验室,江苏苏州215123

出　　处：《西南农业学报》2013年第6期2634-2641,共8页Southwest China Journal of Agricultural Sciences

基　　金：重庆市教委项目(KJ131113);重庆市科委项目(cstc2013jcyjA1202);重庆三峡学院项目(12ZD11;12ZZ31)

摘　　要：为了调查家蚕丝物质生产过程中雌雄之间差异表达基因,本研究构建了家蚕黄茧色限性品种Ysh不同性别的丝腺与中肠LongSAGE文库,按照P<0.05和差异倍数大于2倍的标准,筛选出差异标签,借助家蚕全基因组公共数据库,通过参考文库、GLGI等方法对这些标签进行注释。结果表明,雌性和雄性SAGE文库分别获得了96 713和98 126个标签次数,共筛选出了202个差异标签,其中108个标签在雌性中上调表达,94个标签在雄性中上调表达,最后有76个标签得到了基因注释,认为是家蚕5龄期幼虫雌雄之间差异表达的基因。本SAGE文库中提供的大量性别差异标签对于进一步阐明限性黄茧品种雌雄差异性状机理具有一定的参考价值。In order to investigate genes differentially expressed between the sexes during silk biosynthesis of domestic silkworm （ Bombyx mori）, two Long-SAGE libraries were constructed from the midguts and silkglands of both males and females of a sex-limited strain using 5th instar larvae. Differently expressed tags were selected based on P 〈 0.05 and at least a 2.0-fold change between sexes and annotated by silkworm genome-wide public database, reference library and GLGI method. The results showed that female and male SAGE library had 96,713 and 98,126 SAGE tags, respectively. Among these tags, 202 were differentially expressed tags, of which 108 tags were up-regulated in females while 94 tags were up-regulated in males. Overall, 76 genes were then annotated and considered the SAGE-extracted genes that were differentially expressed between the sexes of the whole 5th instar larvae. The established SAGE library would contribute to the further identification of genes related to sexual disparity in yellow cocoon sex-limited strain of the silkworm,Bomby.x mori.

关 键 词：家蚕 黄茧限性品种 LongSAGE 

分 类 号：S851.347.31[农业科学—预防兽医学]