烤烟品种‘Oxford207’青枯病抗性的遗传分析与分子标记初选  

Inheritance of Resistance to Ralstonia solanacearum in Flue-cured Tobacco‘Oxford207' and Molecular Marker Screen

作　　者：范江[1,2] 刘勇[1,2] 童治军[1,2] 李永平[1,2] 

机构地区：[1]云南省烟草农业科学研究院 [2]中国烟草育种研究南方中心,昆明650021

出　　处：《中国农学通报》2013年第34期50-55,共6页Chinese Agricultural Science Bulletin

基　　金：中国烟草总公司云南省公司科技项目"烟草马铃薯Y病毒病和青枯病抗性遗传分析与转育"(2010YN01);云南省社会发展科技计划"烟草马铃薯Y病毒病抗性遗传分析"(2010CD127)

摘　　要：为了研究烟草青枯病抗性的遗传特性和开发抗性相关分子标记,以抗青枯病的烟草品种‘Oxford207’,感病品种‘红花大金元’为亲本,构建了F1、F2和BC1群体,并采用苗期恒温水培接种法鉴定了群体青枯病的抗性。结果表明,接种后定期调查的F1、F2和BC1F1的死亡率比较接近,均稳定介于抗病亲本和感病亲本之间。F1、F2和BC1F1死亡率曲线下面积比较接近,同样介于抗病与感病亲本之间。表明‘Oxford207’的青枯病抗性不符合典型的显性或隐性基因控制模型,属于加性基因控制。采用简单重复序列(SSR)和分离群体分组分析法初步筛选了抗性相关的分子标记。从800多条SSR引物中,筛选出263个在抗感亲本间有多态性且在F1中呈共显性的引物、3个在抗感池之间有多态性的引物。In order to evaluate inheritance of resistance to Ralstonia solanacearum of tobacco and developing molecular markers, tobacco variety‘Oxford207＇and ‘Honghuadajingyuan＇ （HD）, which resistant to bacterial wilt （BW） and susceptible to BW respectively were used as parents. F1, F2, BC1 populations were constructed.The resistance of parents and populations were evaluated by temperature-controlled water inoculation R.solanacearum at seedling stage. The death rates of F1, F2 and BC1F1 were similar, which were steadily between resistant and susceptible parents＇ regular surveys. The area under the disease curve of F1, F2and BC1F1 were similar, which were steadily between resistant and susceptible parents＇ also. Those data indicated that the resistance of‘Oxford207＇ to bacterial wilt was additive, not typical dominant or recessive. Simple sequence repeat （SSR） technology and bulked segregant analysis were applied to identify markers linked to the resistance gene. Among more than 800 pieces of SSR primers, 263 primers had polymorphism between resistant and susceptible parents and showed co-dominant in F1, 3 primers had polymorphism between the resistant pool and susceptible pool of F2 individual plants.

关 键 词：烟草 青枯病 抗性遗传分析 SSR 

分 类 号：S572[农业科学—烟草工业]