中国石蒜花粉活力测定研究  

Studies on the Vitality of the Pollen of Lycoris chinensis

作　　者：陈默[1] 郑玉红[1] 陆波[1] 徐敏[1] 彭峰[1] 

机构地区：[1]江苏省中国科学院植物研究所/南京中山植物园,南京210014

出　　处：《中国农学通报》2013年第34期122-126,共5页Chinese Agricultural Science Bulletin

基　　金：2011年江苏省农业科技自主创新资金项目"特色球根花卉石蒜的新品种培育"[cx(11)1016];2011年江苏省科技支撑计划"石蒜属植物的标准化快繁和栽培研发示范"(BE2011440)

摘　　要：为了解中国石蒜的花粉活力及其最佳体外萌发条件,分别采用TTC染色法和离体萌发法对中国石蒜的新鲜花粉的活力进行了测定。pH 7.0,5%TTC在35℃条件下染色结果显示99%的中国石蒜花粉具有活力。对影响中国石蒜花粉萌发和花粉管伸长的3个因素(蔗糖、硼酸和氯化钙)进行单因素实验,发现三者的最适浓度分别为蔗糖150 g/L、硼酸60 mg/L、氯化钙50 mg/L。3种浓度的培养液中,中国石蒜的花粉萌发率分别为36.7%、77.6%和44.2%。在中国石蒜花粉萌发最适蔗糖浓度(150 g/L)的基础上,正交设计硼酸和氯化钙浓度,实验结果表明,其最适浓度与单因素实验相同,但花粉萌发率达87%,花粉管伸长量达897μm,远远高于单因素实验的结果,表明三者协同作用,促进中国石蒜花粉的萌发和花粉管的伸长。上述实验证实中国石蒜花粉具有较高的活力。为中国石蒜与其他石蒜属植物的种间杂交可行性提供了实验依据。In order to understand the viability and the conditions of the pollen germination in vitro of Lycoris chinensis, the viability of fresh pollen of Lycoris chinensis were determined by TTC staining method and in vitro culture method. 99% pollen had vitality in 5% TTC solution at the temperature of 35℃, pH 7.0. The single test of the three factors, concentrations of sucrose, boric acid and CaCl2which effects the pollen germination and the pollen tube elongation were conducted. The most suitable concentration of them was150 g/L, 60 mg/L and 50 mg/L. And the pollen germination in them was 36.7%, 77.6% and 44.2%,respectively. When the sucrose concentration was 150 g/L, orthogonal designed that of boric acid and CaCl2.The results showed the most suitable concentrations for the pollen germination and the pollen tube elongation were the same as the single factor test, but the pollen germination rate was 87% and the pollen tube length was897 μm, which was much higher than that. This suggested that there was a synergistic effect between sucrose,boric acid and CaCl2, which promoted the pollen germination and the pollen tube elongation. All the data proved the pollen of L. chinensis had high vitality and offered the experimental base for the inter-species hybridization of L. chinensis and other species in Lycoris Herb.

关 键 词：中国石蒜 生命力 花粉萌发 花粉管伸长 

分 类 号：Q944.42[生物学—植物学]