大豆体细胞胚胎组织培养及遗传转化研究进展  

The Research Process on Somatic Embryo Tissue Culture and Genetic Transformation in Soybean

作　　者：何禹璇 郭东全[2] 钱雪艳[2] 杨向东[2] 李闯[2] 董英山[2] 王丕武[1] 

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林省农业科学院农业生物技术研究所,长春130033

出　　处：《中国农学通报》2013年第36期21-27,共7页Chinese Agricultural Science Bulletin

基　　金：国家转基因生物新品种培育重大专项"抗病虫转基因大豆新品种培育"(2011ZX08004-004)

摘　　要：大豆体细胞胚胎遗传转化体系是提高外源基因遗传稳定性,克服嵌合体的有效途径,因此建立高效稳定的大豆体细胞胚胎组织培养体系并广泛应用于大豆的遗传转化是完善大豆遗传转化研究的重要研究内容。本研究对目前大豆体细胞胚胎再生体系的组织学和遗传转化的研究进展进行了总结,分析了大豆体细胞胚胎组织培养和遗传转化的影响因素。应在现有的组织学研究的基础上,充分考虑影响大豆体细胞胚胎诱导的因素,对遗传转化方法进行改良,以期为大豆体细胞胚胎遗传转化体系提供新的研究思路。The system of soybean somatic embryogenesis genetic transformation is the effective way to improve the genetic stability of exogenous gene and overcome the chimera. Therefore establish a highly efficient and stable soybean somatic embryonic tissue culture system and widely used in the genetic transformation of soybeans, which is the important research content to improve the soybean genetic transformation. This article summarized the histology of soybean somatic embryogenesis regeneration system and genetic transformation progress, and analyzed the influence factors of tissue culture embryogenesis regeneration system. It should be on the and genetic transformation on the soybean somatic basis of the existing histological research, fully considering the influencing factors of soybean somatic embryo induction and improving the genetic transformation methods, in order to provide a new research idea for the soybean somatic embryo genetic transformation system.

关 键 词：体细胞胚 大豆 受体系统 转基因方法 

分 类 号：Q78[生物学—分子生物学]