安徽省四地市水稻细菌性条斑病对链霉素的抗药性监测  

Monitoring of Streptomycin Resistance in Xanthomonas oryzae pv.oryzicola in Four Cities of Anhui Province

作　　者：李云飞[1,2] 陈雪娇[1] 檀根甲[2] 姚剑[1] 

机构地区：[1]安徽出入境检验检疫局,合肥230022 [2]安徽农业大学,合肥230036

出　　处：《中国农学通报》2013年第36期347-350,共4页Chinese Agricultural Science Bulletin

基　　金：安徽检验检疫局项目"水稻黄单胞菌检测方法的研究"(AHKT-01-2011);安徽检验检疫局项目"出口杂交稻中水稻干尖线虫检疫处理技术研究"(AHKT-01-2012)

摘　　要：于2010年采集安徽省滁州、安庆、宣城和巢湖4个城市水稻细菌性条斑病菌108株,以及2011年水稻细菌性条斑病菌168株,测定其对链霉素的抗药性。从这4个城市的发病叶片样本中分离纯化病原菌,并采用区分剂量法,配置含有10μg/mL链霉素的NA平板,能够在含药平板上生长的菌株为抗药性菌株,不能生长的为敏感菌株。从病原菌样本中分离纯化水稻细菌性条斑病菌,总的分离率为45.10%;抗药性监测结果表明,2010—2011年采自安徽省4个城市水稻细菌性条斑病菌在含10μg/mL链霉素的NA平板上不能生长,因此被认为是对链霉素的敏感菌株,未发现抗药性菌株。虽然未分离到田间对链霉素抗药性的水稻细菌性条斑病菌菌株,但由于链霉素具有较高的抗药性风险,因此对于该病菌对链霉素的抗药性仍需要连年进行。During 2010 and 2011, a total of 108 and 168 single-colony strains of Xanthomonas oryzae pv. oryzicola were obtained from diseased rice leaves which were collected from four cities of Anhui Provinces including Chuzhou, Anqing, Xuancheng and Chaohu, respectively. The sensitivity of these strains to streptomycin was tested by the discriminatory dosage method. The strains were generated from the diseased samples of rice from the four cities. The strains, which can grow on the NA plates amended with 10 }~g/ml streptomycin, were considered resistant, or, they were considered to be sensitive ones. The overall ratio of the generation of these strains was 45.10%. The results of the resistance monitoring showed that almost all the X. oryzae pv. oryzicola strains in Anhui Province during 2010-2011 were sensitive to streptomycin and no streptomycin-resistant strains were found in the four surveyed cities. Although no strains collected from fields were resistant to streptomycin were collected, streptomycin possesses high resistance risk. Therefore, monitoring to streptomycin should be continued in the next years.

关 键 词：水稻细菌性条斑病菌 链霉素 抗药性 监测 

分 类 号：S482.2[农业科学—农药学]