一种生物诱抗剂防治黄瓜白粉病效果及对诱导酶的影响  

Effect of a Biological Resistance Inducer on Controlling Cucumber Powdery Mildew and Inducing Enzymes Activities

作　　者：刘琴[1,2] 吴毅歆[3] 薛原[1] 毛自朝[3] 黄莲英[4] 何月秋[1,3] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程研究中心,昆明650201 [2]云南农业职业技术学院,昆明650031 [3]云南农业大学农学与生物技术学院,昆明650201 [4]云南省玉溪市农业局,云南玉溪653100

出　　处：《中国农学通报》2013年第36期367-371,共5页Chinese Agricultural Science Bulletin

基　　金：国家高技术研究发展计划(863计划)(2011AA10A205)

摘　　要：为了明确生物诱抗剂对黄瓜白粉病的防治效果及抗性相关诱导酶的影响，通过盆栽接种实验测定防效和诱导酶活性测定，以感病品种‘新津研4号’为试材，通过实验室制作的一种生物诱抗剂（biologicalresistanceinducer，BRJ）为诱导剂，研究了诱导剂对白粉病的预防效果以及抗性诱导过程中一些抗病相关物质的变化、酶活性的变化。结果表明，施用诱抗剂浓度越高和次数越多，防病效果越好。喷施诱抗剂10、20、100μg/mL24h后，接种病菌72h后，防病效果分别达59．91％、85．02％和89．43％；每隔24h喷施100μg／mL，1次、2次、3次和4次，72h后，防治效果分别达80．32％、98．23％、98．30％和100．00％。诱抗剂提高了黄瓜叶片中的POD、SOD、PAL和CAT等防御相关酶活性，至第5～7天仍然保持很高的水平，其中PAL活性至第9天仍达空白对照的4．37倍。To understand the effect of a biological resistance inducer（BRI） on controlling cucumber powdery mildew and inducing enzymes activities, the potted, inoculation and POD, SOD, PAL and CAT tests were proceeded. In this study, a biological resistance inducer （BRI） was used to induce successfully cucumber resistance to powdery milde and to understand the resistance mechanism. Cucumber was pot planted and inoculated with BRI to study Pathogenesis-related enzymatic activity and the control effect to powdery mildew. The results revealed that the control effects of cucumber powdery mildew were 59.91%, 85.02% and 89.43% after 72 hours when 10, 20, 100 μg/mL of BRI were sprayed onto cucumber seedlings before 24 hours when the pathogen was inoculated and 80.32%, 98.23%, 98.30% and 100.00% after 72 h when 100 μg/mL of BRI were sprayed 1, 2, 3 and 4 times before the pathogen was inoculated. BRI increased the activities of the defense-related enzymes, POD, SOD, PAL and CAT of cucumber leaf until 5-7 days after its spray, especially PAL activity was as 4.37 times high as the negative control without any sprays at the 9th day.

关 键 词：生物诱抗剂 黄瓜白粉病 诱导抗性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]