紫癜性肾炎患者外周血单个核细胞Toll样受体3和4的信号转导途径  

Toll-like receptor 3 and Toll-like receptor 4 signal transduction pathways in the peripheral blood mononuclear cells of purpura nephritis patients

作　　者：付元[1] 刘秀琴[2] 程娜[1] 张守青[1] 常红[1] 张秋业[1] 陈秀霞[1] 高兴娟[3] 

机构地区：[1]青岛大学医学院附属医院儿内科,山东省青岛市266003 [2]青岛市市立医院儿内科,山东省青岛市266003 [3]烟台市毓璜顶医院儿内科,山东省烟台市264000

出　　处：《中国组织工程研究》2013年第53期9182-9188,共7页Chinese Journal of Tissue Engineering Research

基　　金：山东省优秀中青年科学家科研奖励基金(BS2011SW006)~~

摘　　要：背景:目前关于Toll样受体3和Toll样受体4介导的信号转导通路在紫癜性肾炎的发病机制中的作用尚不清楚。目的:分析Toll样受体3和Toll样受体4在过敏性紫癜和紫癜性肾炎发病机制中的作用。方法:选取过敏性紫癜患儿64例,分为过敏性紫癜无肾损害组36例及过敏性紫癜性肾炎组28例,另选健康儿童30例作为正常对照组。实时荧光定量PCR检测外周血单核细胞Toll样受体3、Toll样受体4、髓样分化蛋白2、髓样细胞分化因子88、白细胞介素1β、白细胞介素6、白细胞介素12 mRNA的基因相对表达量;应用流式细胞术检测外周血单核细胞Toll样受体3、Toll样受体4蛋白表达率。结果与结论:①过敏性紫癜患儿Toll样受体4 mRNA及蛋白表达显著高于正常对照组(P<0.05)。紫癜性肾炎组Toll样受体4 mRNA及蛋白表达均显著高于紫癜无肾损害组(P<0.05)。②过敏性紫癜组髓样分化蛋白2、髓样细胞分化因子88、白细胞介素1β、白细胞介素6 mRNA的表达均显著高于正常对照组(P<0.05),白细胞介素12 mRNA的表达显著低于正常对照组(P<0.05);紫癜性肾炎组髓样分化蛋白2、髓样细胞分化因子88、白细胞介素1β、白细胞介素6 mRNA的表达显著高于紫癜无肾损害组(P<0.05),紫癜性肾炎组白细胞介素12 mRNA的表达显著低于紫癜无肾损害组(P<0.05)。③过敏性紫癜组患儿外周血单核细胞Toll样受体4 mRNA与蛋白表达呈正相关(r=0.60,P<0.01);过敏性紫癜患儿Toll样受体4 mRNA与髓样分化蛋白2、髓样细胞分化因子88、白细胞介素1β、白细胞介素6表达均呈正相关(P<0.01),与白细胞介素12 mRNA表达呈负相关(r=-0.66,P<0.01)。提示Toll样受体4可能通过髓样细胞分化因子88依赖信号转导途径介导过敏性紫癜的免疫发病机制,Toll样受体4的过度活化可能与过敏性紫癜的肾损伤有关。BACKGROUND： Toll-like receptor 3 and Toll-like receptor 4 may be play a significant role in the pathogenesy of many diseases about the renal, but, at present, the influence of Toll-like receptor 3 and Toll-like receptor 4 signal transduction pathway in children with Henoch-Sch0nlein purpura nephritis has been unknown. OBJECTIVE： To investigate the mechanism of Toll-like receptor 3 and Toll-like receptor 4 in Henoch-SchOnlein purpura and Henoch-Sch^nlein purpura nephritis. METHODS： Totally 64 children with a clinical diagnosis of Henoch-Sch6nlein purpura were enrolled in the study and divided into two groups, 36 children with non-Henoch-SchOnlein purpura nephritis and 28 children with Henoch-SchOnlein purpura nephritis. Another 30 healthy age-matched children served as controls. The relative expression levels of Toll-like receptor 3, Toll-like receptor 4, myeloid differentiation protein 2, myeloid differentiation factor 88, interleukin-ll3, interleukin-6, interleukin-12 mRNA in peripheral blood mononuclear cells were detected by real-time fluorescent PCR. Protein levels of Toll-like receptor 3 and Toll-like receptor 4 were detected by flow cytometric analysis. RESULTS AND CONCLUSION： （1） Compared with the control group, the relative expression levels of Toll-like receptor 4 mRNA and protein were remarkably increased in children with Henoch-SchOnlein purpura （P 〈 0.05）; compared with the children with non-Henoch-Sch5nlein purpura nephritis, the relative expression levels of Toll-like receptor 4 mRNA and protein were remarkably increased in children with Henoch-Sch6nlein purpura nephritis （P 〈 0.05）. （2） Compared with the control group, mRNA expression levels of myeloid differentiation protein 2, rnyeloid differentiation factor 88, interleukin-113, and interleukin-6 were remarkably increased in children with Henoch-Sch6nlein purpure （P 〈 0.05）, while interleukin 12 mRNA expression level was remarkably decreased （P 〈 0.05）. Compared with the children with non-Henoch-SchOnlein p

关 键 词：器官移植 器官移植基础实验 过敏性紫癜 紫癜性肾炎 单个核细胞 TOLL样受体3 Toll样受体4 髓样细胞分化因子88 髓样分化蛋白2 白细胞介素类 省级基金 

分 类 号：R318[医药卫生—生物医学工程]