Dlx1的天然反义转录物在大脑发育中的功能探索  

Role of Dlx1 Natural Antisense Transcript in Mice Brain Development

作　　者：吴朝[1,2] 赵阿妮[1,2] 朱丽媛[1,2] 阴彬[1,2] 张靖[1,2] 

机构地区：[1]中国医学科学院基础医学研究所 [2]北京协和医学院基础学院生物化学与分子生物学系医学分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2013年第6期607-610,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(31071203)~~

摘　　要：目的探索Dlx1的天然反义转录物(Dlx1as)在小鼠大脑发育过程中的功能。方法根据生物信息学分析与分子生物学实验验证Dlx1as是否具有多聚腺苷酸尾,采用实时定量PCR检测Dlx1as与Dlx1 mRNA在小鼠大脑发育过程中随时间的变化,利用原位杂交的技术比较Dlx1as与Dlx1 mRNA空间表达谱。结果 Dlx1as具有多聚腺苷酸尾,在小鼠的胚胎发育过程中,从E14.5到E18.5高表达,与Dlx1 mRNA在各时间点表达水平虽比较接近,但在表达区域存在互补的特点。结论 Dlx1as在小鼠大脑发育过程中有一段时间的高表达,与蛋白编码基因Dlx1 mRNA空间互补,推测其可能是通过调控Dlx1 mRNA来影响小鼠的大脑发育。Objective To explore the role of Dlxl as, a natural antisense transcript, in mice brain de- velopment. Methods Based on the bioinformatic and molecular biological findings, we confirmed whether the Dlxl as has a poly-A tail. The temporal expressions of Dlxl as and Dlxl mRNA were determined by quantitative re- al-time PCR and the spatial expressions of Dlxlas and Dlxl mRNA were compared by using in situ hybridiza- tion. Results Dlxl as had a poly-A signal. Dlxl as＇ s expression was at a high level through a period of time in the embryo development, which was similar with Dlxl mRNA in temporal but different in spatial. Conclusions Dlxlas is highly expressed in a certain period during mice brain development, which spatially supplements the mRNA expression of D1 xl. Therefore, it is supposed that Dlxl as influences the mice brain development by regu- lating Dlxl mRNA.

关 键 词：Dlx1as 小鼠大脑发育 天然反义转录物 原位杂交 

分 类 号：Q752[生物学—分子生物学]