神经病理性疼痛相关蛋白质在双侧慢性缩窄性损伤大鼠脊髓后角的表达  

Expressions of Neuropathic Pain-related Proteins in the Spinal Cord Dorsal Horn in Rats with Bilateral Chronic Constriction Injury

作　　者：申乐[1] 李旭[1] 王海棠[1] 虞雪融[1] 黄宇光[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院麻醉科,北京100730

出　　处：《中国医学科学院学报》2013年第6期628-633,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(31070930、81200869);北京协和医院青年科研基金(2009124)~~

摘　　要：目的评估双侧慢性缩窄性损伤(CCI)大鼠的疼痛行为学改变,分析鉴定双侧CCI大鼠神经病理性疼痛相关蛋白的表达情况。方法以雌性SD大鼠为研究对象,采用双侧坐骨神经松结扎法制造双侧CCI模型,电子von Frey和丙酮刺激检测机械痛阈与冷痛阈,液相色谱-质谱/质谱联用(LC-MS/MS)技术分析鉴定CCI大鼠脊髓后角神经病理性疼痛相关蛋白质的表达情况。结果双侧CCI大鼠术后第7和14天的机械痛阈和冷痛阈较假手术组与正常组大鼠显著降低(P均<0.05)。双侧CCI大鼠与假手术大鼠比较,共发现25种差异表达蛋白质,其中表达上调18种,表达下调7种。结论双侧CCI大鼠的机械痛阈与冷痛阈显著降低,发现其脊髓后角存在25种与疼痛行为学相关的蛋白质。Objective To evaluate the pain-related behavioral changes in rats with bilateral chronic constriction injury （bCCI） and identify the expressions of neuropathic pain-related proteins. Methods The bCCI models were established by ligating the sciatic nerves in female Sprague Dawley rats. Both mechanical hyperalgesia and cold hyperalgesia were evaluated through electronic yon Frey and acetone method. Liquid chromatography- mass spectrometry/mass spectrometry was applied to characterize the differentially expressed proteins. Results Both mechanical withdrawal threshold and cold hyperalges day 7 and 14, when compared with na＇l＇ve or sham rats （ ia threshold decreased significantly on the postoperative P 〈 0. 05 ） . Twenty five differentially expressed pro- teins associated with bilateral CCI were discovered, with eighteen of them were upregulated and seven of them downregulated. Conclusions The bCCT rats have remarkably decreased mechanical and cold hyperalgesia thresholds. Twenty five neuropathic pain-related proteins are found in the spinal cord dorsal horn.

关 键 词：双侧慢性缩窄性损伤 机械痛阈 冷痛阈 蛋白质组学 

分 类 号：R614.1[医药卫生—麻醉学]