响应面法优化茶叶多糖提取条件的研究  

Optimization of extraction of polysaccharides from the leaves of camellia sinensis using respond surface method

作　　者：谢亮亮[1] 蔡为荣[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2013年第4期8-11,共4页Journal of Anhui Polytechnic University

基　　金：安徽省国际合作基金资助项目(10080703035)

摘　　要：采用水提法从茶叶中提取粗多糖,采用响应面Box-Behnken实验设计方法对提取时间、液固比和提取温度进行优化,从而获得最佳的多糖提取工艺.采用多元回归分析对实验数据进行拟合对并其进行统计学方差分析,得最佳提取条件为:提取时间106min,液固比30,提取温度64℃.在此优化条件下,粗多糖得率为13.26%,与预测值13.14%非常接近.The crude polysaccharides were extracted from the leaves of Camellia sinensis using water ex- traction method. Respond surface method based on Box-Behnken design was employed to obtain the best polysaccharides extraction process parameters.extraction time, water to the raw material ratio and ex- traction temperature. The experimental data were fitted using multiple regression analysis and also ana- lyzed by appropriate statistical method. The optimum extraction conditions were as follows： extraction time of 106 rain, water to the raw material ratio of 30 and extraction temperature of 64 ℃. Under these conditions, the exnerimental yield was 13.26% ,close the expected value of 13.14%.

关 键 词：响应面 提取时间 液固比 提取温度 

分 类 号：Q815[生物学—生物工程]