蓖麻LEA基因家族的全基因组鉴定、分类及其进化分析  

Genome-wide identification,classification and phylogenetic analysis of LEA gene family in castor bean(Ricinus communis L.)

作　　者：邹智[1] 黄启星[2] 安锋[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《中国油料作物学报》2013年第6期637-643,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31100460);海南省重点科技项目(90107);海南省自然科学基金(312026);中国热带农业科学院橡胶研究所基本科研业务费专项(1630022011014)

摘　　要：基于已公布的基因组和EST数据对蓖麻LEA基因家族进行全面鉴定和系统命名，并在此基础上分析了其基因结构、生化特征、进化关系和表达特性。结果显示，蓖麻基因组中存在27个LEA基因，分属于LEA_1、LEA_2、LEA_3、LEA_4、LEA_5、LEA_6、dehydrin和SMP 8个亚家族，它们散布于24条scaffold上，含有0～2个内含子不等，且其中有3个基因存在可变剪接；根据编码蛋白中的Pfam结构域类型及其进化关系，将这些LEA基因依次命名为RcLEA1-1和-2、RcLEA2-1和-2、RcLEA3-1至-3、RcLEA4-1至-7、RcLEA5-1和-2、RcLEA6-1和-2、RcLEA7-1至-5和RcLEA8-1至-4；利用BLAT分析基因表达谱显示，在叶片、花、II/III期胚乳、V/VI期胚乳和种子等组织中，所有RcLEA基因都有表达；启动子分析表明，RcLEA基因的启动子区富含LTRE、ABRE、MYC、MYB和W-box等逆境应答相关顺式作用元件。Late embryogenesis abundant （LEA） proteins, a class of extremely hydrophilic proteins which were first identified in plant embryogenesis, were also found in vegetative tissues. They play important roles in plant desiccation tolerance. In this study, a genome-wide search was carried out to identify LEA genes in castor bean （Ricinus communis L.）, a Euphorbiaceae plant that is well known for its plasticity to unfavorable environments. As a result, a total of 27 RcLEAs representing 8 groups were identified, 3 of which were found to have alternative splicing isoforms. Based on protein characteristics and phylogenesis, the 27 RcLEAs contained 0 to 2 introns and distributed across 24 released scaffolds, and were named as RcLEA1-1 and -2, RcLEA2-1 and -2, RcLEA3-1 to -3, RcLEA4-1 to -7, RcLEA5-1 and -2, RcLEA6-1 and -2, RcLEA7-1 to -5 and RcLEA8-1 to 4. Using the BLAT method, all RcLEA genes were expressed in at least one of the examined tissues such as leaf, flower, endosperm_II_III, endosperm_V_VI and seed. Their promoters enriched stress-responsive cis-acting elements including LTRE, ABRE, MYC, MYB and W-box.

关 键 词：蓖麻 全基因组 LEA基因家族 分类 进化分析 

分 类 号：S565.603[农业科学—作物学]