基于磁性微球与鲁米诺负载化学发光脂质体的生物传感器检测癌胚抗原  

Study on the detection of CEA based on magnetic beads with luminol-encapsulated liposome chemiluminescenceassay

作　　者：严喜鸾[1] 肖义陂[2] 鲁卢 陈静雯[1] 肖鉴谋[1] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031 [2]南昌市洪都中医院,江西南昌330008

出　　处：《南昌大学学报（理科版）》2013年第5期464-468,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金青年科学基金资助项目(81102289);江西省自然科学基金资助项目(20132BAB205106);江西省教育厅青年科学基金资助项目(GJJ10053)

摘　　要：以磁性微球作为分离和富集载体,构建了鲁米诺化学发光脂质体的生物传感器并实现癌胚抗原（carcinoembryonic antigen CEA）的高灵敏分析检测。实验考察了鲁米诺脂质体制备方法及鲁米诺脂质体在60h内的稳定性,并优化了检测条件。结果表明CEA的测定线性范围为1-700ng·mL-1,检出最低浓度达1ng·mL-1。该方法无需标记、快速、简便、灵敏,为癌胚抗原的高灵敏度检测提供了新的方法。A luminol-encapsulated liposome chemiluminescence biosensor for sensitive determination of CEA was developed based on magnetic microspheres as immobilizing and separating carrier.The best preparation method of luminol-encapsulated liposomes was investigated and the prepared luminol-encapsulated liposomes had good stability in 60hours days.Under the optimized detection conditions,a linear relationship is found between CEA concentration and the CL intensity from 1ng/mL to 700ng·mL-1.The lowest detectable concentration of CEA was 1ng·mL-1.Grounded on the simple,fast and high sensitive analysis.this lable-free protocol would be a potential enlarging tool for the determination of other bio-analytes.

关 键 词：脂质体 CEA 磁性微球 化学发光分析 

分 类 号：TQ5-33[化学工程]