雌性大鼠尿道括约肌损伤修复过程中机械生长因子mRNA表达的改变  

Urethral Sphincter Injury and Repair of Mechano Growth Factor mRNA Expression in the Female Rats

作　　者：王娟[1] 王志莲[2] 郝敏[2] 

机构地区：[1]山西医科大学研究生学院,太原030001 [2]山西医科大学第二临床医院妇产科

出　　处：《国际妇产科学杂志》2013年第6期558-560,共3页Journal of International Obstetrics and Gynecology

摘　　要：目的:观察雌性未育Wistar大鼠尿道括约肌损伤后修复过程中局部内源性机械生长因子(MGF)mRNA的表达情况,进一步探讨MGF是否参与尿道括约肌损伤后肌细胞再生修复过程。方法:雌性未育Wistar大鼠60只随机分为2组,一组模拟产伤制备尿道括约肌损伤模型作为实验组,共30只,分别于损伤后的第1,4,7,10,14天各处死6只,取出尿道;另一组30只为尿道未损伤组作为空白对照组,在与实验组相同时间点处死,取出尿道。行内参法的相对定量荧光聚合酶链反应(PCR)检测局部MGF表达情况。结果:空白对照组大鼠局部内源性MGF mRNA只有极少量的表达,实验组在损伤后第1天表达量即显著增加,后逐渐升高,到第7天达高峰,之后逐渐下降,但在第14天表达量仍高于正常组(P<0.05)。结论:雌性大鼠尿道括约肌损伤修复过程中局部MGF mRNA表达量增加,且损伤时间不同表达量有所变化,提示MGF可能参与了尿道括约肌的损伤修复。Objective：To observe the expression situation of the partial endocardial mechano growth factor mRNA in the recovery process of the injured female WISTAR rats＇ urethra sphincter who have childless show whether the machinery growth factor does participate in muscle cell regeneration and recovery process of Wistar rats＇ urethra sphincter. Methods：Divide 60 female Wistar rats who have childless into two groups stochastically. In the experimental group, there are 30 of them whose urethra sphincters are injured like birth injury by obstetrician＇s instruments. And then execute them and take out the urethra on the 1st, 4th, 7th, l Oth and 14th day respectively （6/d）. There are 30 as the blank control group, Execute them and take out the urethra, too. On all specimens,the comparative quantitation PCR of internal reference should be carried on,then examine the expression situation of the partial mechano growth factor. Results：In the blank control group, the partial endocardial machinery growth factor mRNA only has extremely little expression quantity, but the expression of it increases significantly on the 1 st day after the urethra sphincter was injured,and increases gradually,then reaches the peak on the 7th day,and then drops gradually. However,the expression quantity on the 14th day is still higher than that in the blank control group （P〈0.05）. Conclusions ：The expression quantity of the partial endocardial machinery growth factor mRNA increases in the recovery process of the injured female Wistar rats＇ urethra sphincter who have childless,and the expression quantity is different in different injured time. It shows that the machinery growth factor doe＇s participate in muscle cell regeneration and recovery process of Wistar rats＇ urethra sphincter.

关 键 词：尿失禁 压力性 胰岛素样生长因子Ⅰ 机械生长因子 尿道括约肌 

分 类 号：R694.54[医药卫生—泌尿科学]