广枣总黄酮对体外培养大鼠心脏成纤维细胞Ⅰ,Ⅲ型胶原mRNA及蛋白表达的影响  

In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type Ⅰ and type Ⅲ mRNA and protein of cultured rat cardiac fibroblasts

作　　者：包俊萍 金明 杨雨民 高晓慧 舒亮[3] 邢慧慧[3] 贾磊[3] 

机构地区：[1]内蒙古自治区中医医院心内科,内蒙古呼和浩特010020 [2]内蒙古林业总医院中医科,内蒙古牙克石022150 [3]内蒙古医科大学研究生学院,内蒙古呼和浩特010050

出　　处：《药学学报》2014年第1期136-141,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81060362);内蒙古自治区自然科学基金资助项目(2009BS1204)

摘　　要：心肌纤维化（myocardialfibrosis，MF）常伴随风湿性心脏病、高血压、心肌梗死及心力衰竭等多种心血管疾病，在增龄和冠状动脉粥样硬化过程中也会发生MF。MF又称为心脏胶原网络重构，以心肌胶原浓度的显著升高或心肌胶原容积分数的明显增加为特征，This study aims to investigate the effect of total flavones of Fructus Chorspondiatis （TFFC） on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts （CFs） induced by angiotensin II （Ang II）, and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats （1-3 d after birth）. The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.

关 键 词：广枣总黄酮 I型胶原 III型胶原 心脏成纤维细胞 血管紧张素II 

分 类 号：R285[医药卫生—中药学]