干细胞微环境力学刺激调节干细胞分化的研究现状  

RESEARCH STATUS OF MECHANICAL STIMULATION OF STEM CELLS DIFFERENTIATION IN STEM CELLS MICROENVIRONMENT

作　　者：崔爽爽[1] 赵文君[1] 于顺禄[1] 邢国胜[1] 赵凤仪[1] 

机构地区：[1]天津医院骨科研究所,天津300211

出　　处：《中国修复重建外科杂志》2014年第1期100-104,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：天津市卫生局科技基金资助项目(2012KY23;2012KY25)~~

摘　　要：目的对干细胞微环境中影响干细胞体外分化的力学刺激研究现状进行综述。方法广泛查阅近年国内外关于干细胞分化研究的文献,对干细胞微环境中影响干细胞分化的各种力学刺激进行综述。结果机械载荷(包括剪应力、机械牵张力、压应力)、细胞培养基质硬度、细胞培养基质纳米形貌、细胞形状等4个方面的力学刺激对干细胞分化的影响是目前研究的热点。微环境力学刺激能够模拟干细胞体内生长的微环境,改变干细胞的形状、大小、排列方向、分化状态及干细胞表面标记物表达,并影响干细胞的谱系分化。结论力学刺激是除化学和生物因素外影响干细胞体外分化的又一主要因素。Objective To review the relative researches about mechanical stimulation of stem cells differentiation in stem cells microenvironment in vitro. Methods The recent related literature about stem cells differentiation in vitro was reviewed and summarized. Results The mechanical loads （including shear stress, mechanical strain, and stress）, substrates stiffness, substrates nanotopography, and cell shape were the 4 important aspects of mechanical factors regulating stem cells differentiation. The mechanical stimulation can simulate the in vivo microenvironment, which can alter the size, shape, alignment, and differentiation state of stem cells, can change the expression of their differentiation markers, and can affect the lineage commitment of stem cells. Conclusion Mechanical stimulation play an important role in regulating stem cells differentiation and cells morphology in addition to chemical and biological factors.

关 键 词：干细胞 微环境 体外分化 力学刺激 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]