广西眼镜蛇毒蛋白Natrin在体外诱导人肝癌细胞SMMC-7721凋亡作用的研究  

Study on induction of apoptosis in human hepatocarcinoma smmc-7721 cells in vitro by Natrin

作　　者：宋慧[1] 徐星新[1] 李映新[1] 李雪华[1] 苏延旭[1] 蒋兴明[1] 梁永红[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《中国药理学通报》2014年第1期118-121,共4页Chinese Pharmacological Bulletin

基　　金：广西区科技厅科学基金资助项目(No桂科自0991127;桂科青0542043)

摘　　要：目的观察广西眼镜蛇毒蛋白Natrin在体外对人肝癌SMMC-7721细胞的增殖抑制作用及凋亡诱导作用。方法以不同浓度的Natrin作用于SMMC-7721细胞24 h后,用MTT法检测细胞的生长抑制率;AO/EB双染色法、透射电子显微镜法观察细胞凋亡、流式细胞仪检测细胞凋亡率及细胞周期。结果 Natrin对人肝癌SMMC-7721细胞有体外抑制作用,随着药物浓度的增大抑制率也随之增大,24 h半数抑制浓度IC50为138.69 mg·L-1;给药后,荧光显微镜下与透射电子显微镜下细胞呈现典型的凋亡形态改变;流式细胞仪检测到随着药物浓度的增大凋亡百分数也随之增大;Natrin能诱导SMMC-7721细胞发生S期和G2/M期阻滞。结论Natrin在体外能抑制人肝癌SMMC-7721细胞增殖并诱导其凋亡。Aim To observe the growth inhibitory effects and apoptosis inducing effects of Natrin on hu- man hepatocarcinoma SMMC-7721 cells in vitro. Methods The growth inhibition ratio of SMMC-7721 cells after exposure to different concentrations of Natrin was measured using MTY assay; morphological changes of apoptosis were detected by AO/EB staining and transmission electron microscopy; the cell cycle and apoptotic percentage were detected using FCM. Re- sults The growth of human hepatocarcinoma SMMC- 7721 cells was inhibited by the treatment of Natrin. With the increase of drug concentration, the growth in- hibitory rate also increased. ICso of 24h was 138. 69 mg · L^-1. After treated by Natrin, typical morphologi- cal changes of apoptosis were observed by fluorescence microscope and transmission electron microscope. With the increase of drug concentration, apoptotic percent- age performed by flow cytometry also increased. Natrin showed the effects of S and G2/M cell cycle arrest. Conclusion Natrin can inhibit proliferation and in- duce apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro.

关 键 词：广西眼镜蛇毒蛋白Natrin 肝癌SMMC-7721细胞 细 胞周期 细胞凋亡 透射电镜 流式细胞术 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]