功能电刺激对脊髓损伤大鼠运动功能恢复和神经再生的影响  

Effects of functional electric stimulation on motor function recovery and nerve regeneration of rats with spinal cord injury

作　　者：王维[1] 马雪[1] 赵晓晨[1] 王文娟[1] 苑秀华[2] 

机构地区：[1]辽宁医学院附属第一医院康复科,锦州121001 [2]中国医科大学附属第一医院康复医学科,沈阳110001

出　　处：《中华神经医学杂志》2014年第1期17-21,共5页Chinese Journal of Neuromedicine

基　　金：辽宁省科技厅社会发展课题（2012408002）

摘　　要：目的探讨功能电刺激（FES）对大鼠脊髓损伤（SCI）后运动诱发电位（MEP）、神经胶质酸性蛋白（GFAV）表达、肌肉湿重的影响。方法选用成年雄性SD大鼠72只，按照随机数字表法分为3组：假手术组、FES组和模型组，每组24只。FES组和模型组大鼠采用NYU打击器制作T9脊髓完全损伤动物模型。FES组损伤后给予后肢大腿位置FES治疗，模型组电极安放后不予通电。假手术组只作椎板切除术，不进行脊髓的损伤。FES后7d、14d、28d、56d，运用诱发电位仪检测各组大鼠的MEP潜伏期、免疫组织化学染色检测GFAP的表达，干-湿重法检测后肢伸肌的湿重。结果FES组14d、28d、56d MEP潜伏期测出，较假手术组明显明显延长，差异有统计学意义（P〈0．05）。28d、56d时FES组大鼠MEP潜伏期较模型组缩短，差异有统计学意义（P〈0．05）。FES组28d、56d时肌肉湿重比率较模型组明显增加，差异有统计学意义（P〈0．05）。电刺激后28d、56d，FES组大鼠GFAP阳性细胞数较模型组大鼠明显减少，差异有统计学意义（P〈0．05）。结论FES在SCI的治疗中不仅能够抑制GFAP的表达，而且能缩短MEP潜伏期,抑制瘫痪肢体肌肉萎缩,促进运动功能恢复。Objective To discuss the effect of functional electric stimulation （FES） on motor evoked potcntial（MEP）, glial fibrillary acidic protein （GFAP） expression and muscle wet weight in rats after spinal cord injury （SCI）. Methods Seventy-two adult male SD rats were chosen and randomly divided into 3 groups： sham-operated group, FES group and model group （n=24）. T9 spinal cord complete injury animal models in the FES group and model group were induced by NYU blow. Rats in the FES group were performed FES at posterior thigh and rats in the model group only placed electrode without electricity. 7, 14, 28 and 56 d after FES, potentiometer test was performed to detect the MEP latency; immunohistochemical staining was employed to detect the GFAP expression; and hind leg extensor muscle wet weight was measured. Results The MEP latency in the FES group was detected 14, 28 and 56 d after FES, which was significantly longer than that in the sham-operated group （P〈0.05）; the MEP latency in the FES group 28 and 56 d after FES was significantly shortened than that in the model group （P〈0.05）. Muscle wet weight in the FES group 28 and 56 d after FES was significantly increased than that in the model group （P〈0.05）. GFAP-positive cells in the FES group 28 and 56 d after FES were significantly decreased than those in the model group （P〈0.05）. Conclusion FES in the treatment of spinal cord injury can not only inhibit the expression of GFAP and shorten MEP latency, but also inhibit paralyzed limb muscle atrophy and promote motor fimction recovery.

关 键 词：脊髓损伤 功能电刺激 神经胶质酸性蛋白 肌肉湿重 运动诱发电位 

分 类 号：R651.2[医药卫生—外科学]