白藜芦醇对大鼠脑缺血再灌注损伤后的神经保护作用研究  

Neuroprotective effect of resveratrol on ischemia/reperfusion injury

作　　者：赵刚[1] 王伟民[1] 杨帅[1] 罗贵聪[1] 刘晓燕[1] 钟世镇[2] 

机构地区：[1]广州军区广州总医院神经外科,广州510010 [2]南方医科大学解剖教研室,广州510515

出　　处：《中华神经医学杂志》2014年第1期26-29,共4页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金（7000055）

摘　　要：目的研究白藜芦醇（Res）对脑缺血再灌注损伤的保护作用和可能机制。方法135只大鼠按照随机数字表法分为9组，每组15只。A组为假手术组，B1、B2组分别为术后持续脑缺血和缺血再灌注后丙二醇溶液组，C1-C3组为持续脑缺血后Res干预组，D1-D3组为缺血再灌注后Res干预组，药物剂量分别为10^-8g／kg、10^-7g／kg、10^-6g／kg。假手术组大鼠只分离右侧颈总动脉及颈内外动脉：脑缺血大鼠采用线栓法制作大脑中动脉栓塞模型：脑缺血再灌注大鼠缺血2h后再灌注；Res干预大鼠于再灌注2h后采用Res治疗3d。所有大鼠采用TTC法测量脑梗死体积，采用干湿重法测定脑含水量，免疫组化染色测定谷氨酸／天冬氨酸转运体（GLAST）及谷氨酸转运体-1（GLT-1）蛋白表达。结果与B组比较，C组和D组中高剂量Res亚组能明显降低大鼠神经功能评分（D3组大鼠评分最低，为1．30±0．481，缩小脑梗死体积（D3组大鼠脑梗死体积比例最低，为16．00％±6．20％），降低脑含水量（D3组大鼠脑含水量最低，为52．30％±8．25％），提高GLAST和GLT-1的表达（D3组大鼠2种蛋白表达量最高，分别为39．98±0．77、171．76±7．22）。结论Res通过提高GLAST和GLT-1的表达，降低梗死区谷氨酸浓度，缩小脑梗死体积和减轻脑水肿来起到脑缺血再灌注损伤后的神经保护作用。Objective To explore whether resveratrol （Res） could effectively suppress infarct size or improve neurological deficits in rat models after acute ischemia or ischemia/reperfusion. Methods One hundred and thirty-five rats were divided randomly into nine groups （n=15）： sham-operated group, ischemia groups, including 0.04% propylene glycol solution treatment group （10^-6 g/kg, B1 group）, low dose of Res intervention group （10^-8 g/kg, C1 group）, middle dose of Res intervention group （10^-7 g/kg, C2 group） and high dose of Res intervention group （10^-6 g/kg, C3 group）, and isehemia/reperfusion groups, including 0.04% propylene glycol solution intervention group （10^-6 g/kg, B2 group）, low dose of Res intervention group （10^-8 g/kg, D1 group）, middle dose of Res intervention group （10-7 g/kg, D2 group） and high dose of Res intervention group （10^-6 g/kg, D3 group）. Rats in the sham-operated group only performed separation of right common carotid artery and internal carotid artery;ischemia was induced by middle cerebral artery occlusion; reperfusion was performed 2 h after ischemia; Res intervention was given for 3 days 2 h after reperfusion. Neurological scale scores of all groups were evaluated, infarct volume was measured by 2,3,5-triphenyltetrazolium chloride （TTC） staining, content of water in brain tissue was measured by wet and dry weight method, and the expressions of glutamate/aspartate transporter （GLAST） and glutamate transport-1 （GLT-1） were observed by immunohistochemical method. Results As compared with B groups, the neurological scale scores in the C2, C3, D2 and D3 group were significantly ameliorated with D3 group having the lowest scores （1.30±0.48）, infarct volume and brain water content were statistically reduced with D3 group having the smallest infart volume （16.00% ±6.20%） and lowest water content （52.30%±8.25%）, and the expressions of GLAST and GLT-1 were significantly increased with D3 group having the highest levels （39.98�

关 键 词：白藜芦醇 脑梗死 谷氨酸 天冬氨酸转运体 谷氨酸转运体-1 

分 类 号：R285.5[医药卫生—中药学]