温和灸对动脉粥样硬化家兔CD40-CD40L轴的影响  

Effects of warm moxibustion on CD40-CD40Laxis in rabbits with atherosclerosis

作　　者：蔡海红[1] 王玲玲[1] 姜劲峰[1] 吴姗[1] 蒋文[1] 

机构地区：[1]南京中医药大学针药结合实验室,江苏南京210029

出　　处：《中国针灸》2014年第1期55-60,共6页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973)项目:2009CB 522905

摘　　要：目的:探索温和灸调脂、抗动脉粥样硬化的作用及其机制。方法:将41只雄性日本大耳白兔随机分为4组,空白组(10只)、模型组(10只)、艾灸组(10只)和药物组(11只)。空白组予普通饲料喂养,其余各组采用高脂饲料喂养联合注射牛血清白蛋白法建立家兔动脉粥样硬化模型。造模后模型组不予治疗干预;艾灸组行温和灸治疗,取双侧"足三里""神阙"穴,每日每穴灸治10min,连续治疗4周;药物组用洛伐他汀胶囊按3.6mg/kg的剂量行灌胃治疗,连续治疗4周。4周后测定总胆固醇(TC)、甘油三酯(TG)等血脂水平及CD40配体(CD40L)、可溶性CD40L(sCD40L)含量、核因子κB(NF-κB)的表达。结果:与模型组比较,艾灸组、药物组均能有效减少总胆固醇、低密度脂蛋白含量(均P<0.05);降低sCD40L水平[模型组(8.310±1.221)ng/mL,艾灸组(7.097±0.846)ng/mL,药物组(7.354±0.631)ng/mL)],降低CD40L的表达[模型组(0.235±0.179)mm2,艾灸组(0.072±0.079)mm2,药物组(0.039±0.015)mm2];NF-κB的表达亦明显减少[模型组(0.145±0.052)mm2,艾灸组(0.052±0.012)mm2,药物组(0.036±0.013)mm2],差异均有统计学意义(P<0.05,P<0.001),艾灸组和药物组比较差异无统计学意义(均P>0.05)。结论:温和灸具有良好的调脂、抗动脉粥样硬化作用,下调CD40-CD40L的表达是艾灸发挥抗动脉粥样硬化作用的可能机制之一。Objective To explore effects and action mechanism of warm moxihustion on regulation of blood li- pids and anti-atherosclerosis. Methods Forty-one male Japanese big-ear white rabbits were randomly divided into a blank group （10 rabbits）, a model group （10 rabbits）, a moxibustion group （10 rabbits） and a medication group （11 rabbits）. Normal diet was applied in the blank group while high-cholesterol diet combined with injection of bo- vine serum albumin were applied in the rest groups to establish rabbit model of atherosclerosis. After establish- ment, the model group was not intervened and warm moxibustion was applied in the moxibustion group at ＂Zusan- li＂ （ST 36） and ＂Shenque＂ （CV 8）, 10 min per acupoint per day for continuous 4 weeks. The medication group was treated with intragastric administration of lovastatin capsule （3.6 mg/kg） for continuous 4 weeks. The level of blood lipids, such as total cholesterol （TC）, triglyceride （TG）, and content of CD40 ligand （CD40L）, soluble CD40 ligand （sCD40L） and expression of nuclear factor NF-~B were tested after 4 weeks. Results Compared with the model group, the moxibustion group and medication group could effectively reduce the contents of TC and low density lipoprotein （all P〈0.05）, lower the level of sCD40L [（8. 310±1. 221） ng/mL in the model group, （7. 097±0. 846） ng/mL in the moxibustion group and （7. 354±0. 631） ng/mL in the medication group], reduce expression of CD40L [-（0. 235±0. 179）mm2 in the model group, （0. 072±0. 079） mm2 in the moxibustion group and （0. 039±0. 015） mm2 in the medication group] and NF-κB [（0. 145±0. 052）mm2 in the model group, （0. 052±0. 012） mm2 in the moxibustion group and （0. 036±0. 013） mm2 in the medication group], indicating the significant difference （P〈0.05,P〈0. 001）. There was no statistical difference between the moxibustion group and medication group （all P〉0.05）. Conclusion The warm moxibustion has great effect

关 键 词：动脉粥样硬化 温和灸 血脂 

分 类 号：R245.8[医药卫生—针灸推拿学]