不同状态下艾灸对穴位局部细胞外钾离子浓度的影响  

Effects of moxibustion on concentration of extracellular potassium ion in acupoint under different status

作　　者：周新异[1] 刘娟[1] 吴巧凤[1] 田晓宁[1] 范亚朋[1] 刘奇[1] 杜萍[1] 唐勇[1] 尹海燕[1] 余曙光[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《中国针灸》2014年第1期61-65,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973)项目:2009CB 522903;四川省教育厅课题:11ZA 055;成都中医药大学校基金项目:ZRYB 201101

摘　　要：目的:观察生理病理两种不同状态下艾灸对穴位局部细胞外钾离子的不同影响,为探索艾灸对穴位局部的作用机制提供实验依据。方法:选取雌性SD大鼠40只,随机分为空白组、空灸组、模型组、模灸组,每组10只。模型组与模灸组采用弗氏完全佐剂造佐剂性关节炎模型(AA模型),其中空白组、模型组不予艾灸干预,空灸组、模灸组艾灸"足三里"30min。各组均用微透析仪于"足三里"穴位局部收集组织液,所得样品均用电解质分析方法进行即时分析,观察"足三里"局部钾离子的浓度变化。结果:①生理状态下(空白组、空灸组),空白组在150min内细胞外钾离子浓度无明显变化(P>0.05);空灸组细胞外钾离子浓度灸前为(1.21±0.31)mmol/L,灸后缓慢升高,60min时达到高峰(2.38±0.42)mmol/L(P<0.05),之后下降,至灸后150min时,钾离子浓度略高于灸前,也略高于空白组,在60min时空灸组与空白组比较,差异有统计学意义(P<0.05)。②病理状态下(模型组、模灸组),模型组细胞外钾离子浓度在150min内无明显变化,各时间点之间差异无统计学意义(均P>0.05);模灸组细胞外钾离子浓度灸前为(1.09±0.12)mmol/L,灸后即有明显升高(1.96±0.18)mmol/L(P<0.05),灸后60min、90mim时虽有下降但仍保持较高水平,分别为(1.87±0.29)mmol/L、(1.59±0.16)mmol/L(均P<0.05);艾灸后的各个时间点模灸组与模型组比较差异均有统计学意义(均P<0.05)。结论:生理状态下艾灸能升高大鼠穴位局部钾离子浓度,但作用时间较短;在病理状态下艾灸大鼠"足三里"穴,局部钾离子浓度明显升高,并且维持时间较长。Objective To observe different effects of moxibustion on extracellular potassium ion in acupoint under physiological and pathological status and provide experimental evidence for exploring action mechanism of moxibustion on acupoint local. Methods Forty female SD rats were randomly divided into a blank group, a blank- moxibustion group, a model group and a model-moxibustion group, 10 cases in each one. The complete Freund＇s adjuvant（CFA） was adopted to establish model of adjuvant arthritis （AA） in the model group and model-moxibustion group. No treatment was given in the blank group and model group while moxibustion was applied at ＂Zusan- li＂ （ST 36） for 30 rain in the hlank-moxibustion group and model-moxihustion group. The tissue fluid in ＂Zusanli＂ （ST 36） was collected with microdialysis and real-time analyzed by electrolytic analyzer. The change of concentra- tion of potassium ion in ＂Zusanli＂ （ST 36） was observed. Results ①Under physiological status, the concentra- tion of extracellular potassium ion in the blank group was not changed within 150 min （P〉0.05） ; before the mox- ibustion, the concentration of extracellular potassium ion in the blank-moxibustion group was （1.21±0.31） mmol/L, and after treatment it was gradually increased and reached its peak at （2.38±0.42） mmol/L after 60 rain （P〈0. 05）, then it was reduced. 150 rain after the treatment, concentration of potassium ion was slightly higher than that before moxibustion as well as that in the blank group. The concentration in the blank-moxibustion group at 60 min was statistically significant compared with that in the blank group （P〈0. 05）. ②Under pathological sta- tus, the concentration of extracellular potassium ion in the model group was not changed within 150 min, differences of which at each time point was not statistically significant （all P〉0.05）. Before the moxibustion, the concentration of extracellular potassium ion was （1.09± 0. 12） mmol/L in the model-moxibustio

关 键 词：艾灸 穴 足三里 细胞外钾离子 穴位局部 

分 类 号：R245.8[医药卫生—针灸推拿学]