帕金森病患者血浆增强α-突触核蛋白寡聚体形成  

Increased formation of α-synuclein oligomers in plasma of patients with Parkinson's disease

作　　者：尹娜[1,2] 陈予东[1,2] 李昕[1,2] 杨巍巍[1,2] 李旭冉[1,2] 于顺[1,2] 

机构地区：[1]首都医科大学宣武医院神经生物学研究室,教育部神经变性病重点实验室,北京100053 [2]北京市老年病医疗研究中心分子诊断实验室,北京100053

出　　处：《首都医科大学学报》2013年第6期840-843,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(81071014);国家重点基础发展计划(973计划)(2011CB504101);首都卫生发展科研专项课题(首发2011-1001-01);国家科技支撑计划课题(2012BAI10B03);北京市自然科学基金(7102076)~~

摘　　要：目的研究帕金森病(Parkinson’s disease,PD)患者血浆对α-突触核蛋白(α-synuclein,α-Syn)寡聚体形成的影响。方法将重组人α-Syn加入PD和对照血浆中,37℃振荡孵育144 h,免疫印迹方法鉴定α-Syn寡聚体形成情况,ELISA方法分析α-Syn寡聚体形成的相对质量浓度。结果α-Syn在PD患者血浆中形成寡聚体含量明显高于对照组。结论 PD患者血浆可促进α-Syn寡聚体形成。Objective To study the effect of plasma of patients with Parkinson＇ s disease （PD） on the formation of oligomeric α- synuclein （ct-Syn）. Methods Recombinant ct-Syn was incubated in the plasma of PD patients and normal subjects. Immunoblotting analysis was used to identify the formation of α-Syn oligomers. A special ELISA method was used to evaluate the amount of α-Syn oligomers formed in PD and control plasma. Results The amount of α-Syn oligomer formed in the plasma of PD patients was higher than that in control plasma. Conclusion Compared with control plasma, the plasma from PD patients enhanced the oligomerization of α-Syn.

关 键 词：帕金森病 血浆 Α-突触核蛋白 寡聚体 

分 类 号：R338[医药卫生—人体生理学]