CD61阳性人脂肪来源细胞体外软骨分化潜能的初步研究  

A Preliminary Study of in Vitro Chondrogenic Potential of Human CD61+Adipose Derived Cells

作　　者：孙恒赟[1,2] 周广东[1,2] 曹谊林[1,2] 

机构地区：[1]北京协和医学院中国医学科学院整形外科医院整形七科,北京100144 [2]上海交通大学医学院附属第九人民医院,上海市组织工程重点实验室,上海200011

出　　处：《中华耳科学杂志》2013年第4期540-542,共3页Chinese Journal of Otology

基　　金：国家自然科学基金(31271046),高等学校博士学科点专项科研基金(20100073110081)

摘　　要：目的利用流式细胞仪分选人脂肪来源细胞(adipose derived cells,ADCs)软骨潜能亚群并初步检测其体外成软骨能力。方法采用酶消化法分离ADCs,以CD61为表面标志,通过流式细胞仪分选纯化,所得CD61+和CD61-两群细胞进行体外成软骨诱导,3周后进行软骨特异性细胞外基质二型胶原免疫荧光染色和软骨特异性基因SOX9和COL II定量PCR检测。结果采用CD61作为分选标志可通过流式细胞仪分选纯化获得两群细胞亚群。免疫荧光染色结果显示两群细胞经软骨诱导后均表达二型胶原。定量PCR结果表明CD61+组SOX9和COL II表达高于CD61-组(p<0.05)。结论 CD61+ADCs具有较强体外软骨分化能力,CD61有望成为ADCs软骨潜能亚群体外分选标志。Objectives To sort out chondrogenic subpopulation of human adipose derived cell （ADCs） with flow cytome- try and preliminarily examine its in vitro chondrogenic potential. Methods ADCs were isolated by enzymes. CD61 ＋ and CD61- subpopulations were sorted out with flow cytometry and chondrogenically induced in vitro. After 3-week induction, col- lagen II, the specific extracellular matrix of cartilage, was examined with immunofluorescent staining and SOX9 and COL II, the specific genes of ehondrocyte, were checked with PCR. Results CD61 could be used as a surface marker for cell sorting of ADCs with flow cytometry. The results of immunofluorescent staining demonstrated that after chondrogenic induction, both of the two cell populations expressed collagen II. Quantitative PCR results showed that the expression of SOX9 and COL II were higher in CD61＋ group than in CD61- group （p〈0.05）. Conclusions CD61＋ ADCs had a strongei chondrogenic differentiation capacity than CD61- cells. CD61 could be used as a specific marker for purifying chondrogenic subpopulation of ADCs.

关 键 词：脂肪干细胞软骨潜能亚群 CD61 分选纯化 

分 类 号：R764.71[医药卫生—耳鼻咽喉科]