Nuclestemin siRNA对人肺腺癌紫杉醇耐药株A549/Taxol的耐药性逆转的研究  

REVERSAL OF DRUG RESISTANCE IN A549 /TAXOL CELLS BY SMALL INTERFERENCE RNA OF NUCLESTEMIN GENE

作　　者：潘艳明[1] 何大业 张伟[3] 任凤云[1] 胡静[1] 

机构地区：[1]牡丹江医学院 [2]牡丹江市肿瘤医院 [3]牡丹江医学院红旗医院,黑龙江牡丹江157000

出　　处：《牡丹江医学院学报》2013年第6期9-11,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生厅科研项目(No.2009-456;No.2007-005)

摘　　要：目的:探讨Nuclestemin基因(NS)特异性RNA干扰对人肺腺癌紫杉醇耐药株A549/Taxol细胞耐药性的逆转作用。方法:NS特异性RNAi表达载体转染A549/Taxol细胞。提取肿瘤细胞总RNA,用RT-PCR方法检测NS的表达;利用MTT法检测紫杉醇的半数抑制浓度(IC50)。结果:NS特异性RNAi表达载体转染A549/Taxol细胞后,NS的表达量明显降低,并且对紫杉醇敏感性的相对逆转率达65.3%。结论:Nuclestemin基因siRNA能有效逆转A549/Taxol细胞的耐药性,明显提高耐药细胞对紫杉醇的敏感性。Objective：To investigate the effect of gene silence of Nuclestemin gene by small interference RNA （siRNA）in drug re- sistant A549/Taxol cell. Methods ： 1. A NS - specific siRNA expression vector was constructed to transfect A549/Taxol cell. 2. Total RNA was extracted from tumor cell, NS expression level was measured by RT - PCR; 50% inhibition concentration （IC50） of paclita- xel on A549/Taxol was determined by MTT method in vitro. Results： The expression level of NS decreased greatly, the relative reversal efficiency of A549/Taxol cell to paelixtel was 65.3%. Conclusion ：The Nuclestemin siRNA can effectively reverse-the drug resistance of A549/Taxol cells, and enhance the the responsiveness of lung cancer to Paelitaxel.

关 键 词：NUCLEOSTEMIN RNA干扰 A549 Taxol细胞 耐药性 

分 类 号：R71[医药卫生—妇产科学]