Influence of extract of Ginkgo biloba leaves tablets on the aquaporin-1 expression in isolated lung ischemia reperfusion  

作　　者：LI Xiang-nan YANG Ji-yao PAN Xue ZHAO Song ZHANG Chun-yang ZHU Deng-yan WANG Peng 

机构地区：[1]Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China [2]Department of Histology and Embryology, Basic Medical College,Zhengzhou University, Zhengzhou, Henan 450001, China, [3]Nursing College of Zhengzhou University, Zhengzhou, Henan 450052, China

出　　处：《Chinese Medical Journal》2013年第24期4720-4723,共4页中华医学杂志（英文版）

摘　　要：Background The extract of Ginkgo biloba leaves tablets, ginaton, is widely used in treating ischemic cerebrovascular disease in the clinic. This study aimed to investigate the expression of aquaporin-1 （AQP-1） in rat lung with ischemia/ reperfusion injury after pretreatment with ginaton, and whether the pretreatment with ginaton reduces the acute lung injury caused by ischemia/reperfusion injury. Methods Adult Wistar rats were divided into two groups. Some rats were used as donors （n=20）, the others as recipients （n=20）. Left lungs of donor rats were used for the isolated lung reperfusion model, which perfused only with low potassium dextran （LPD） solution as group A （n=10）; the others were pretreated with ginaton before reperfusion as group C （n=10）. Right lung of donor rat without any treatment was used as a control group （group B and group D, n=10 for each group）. After the model was established, the expression of AQP-1 in the lung tissues was examined by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction. Results Immunohistochemical examination revealed that AQP-1 was expressed in endothelia. Immunoblotting demonstrated that the relative gray values of AQP-1 protein in groups A and C were 0.65±0.06, 0.88±0.11, respectively. The relative gray values of the mRNA expression in groups A and C were 0.30±0.08, 0.49±0.11, respectively. The expression of AQP-1 protein and mRNA in group C was significantly higher than in group A （P 〈0.05）. Conclusion The pretreatment with ginaton can reduce the acute lung injury caused by ischemia/reperfusion.Background The extract of Ginkgo biloba leaves tablets, ginaton, is widely used in treating ischemic cerebrovascular disease in the clinic. This study aimed to investigate the expression of aquaporin-1 （AQP-1） in rat lung with ischemia/ reperfusion injury after pretreatment with ginaton, and whether the pretreatment with ginaton reduces the acute lung injury caused by ischemia/reperfusion injury. Methods Adult Wistar rats were divided into two groups. Some rats were used as donors （n=20）, the others as recipients （n=20）. Left lungs of donor rats were used for the isolated lung reperfusion model, which perfused only with low potassium dextran （LPD） solution as group A （n=10）; the others were pretreated with ginaton before reperfusion as group C （n=10）. Right lung of donor rat without any treatment was used as a control group （group B and group D, n=10 for each group）. After the model was established, the expression of AQP-1 in the lung tissues was examined by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction. Results Immunohistochemical examination revealed that AQP-1 was expressed in endothelia. Immunoblotting demonstrated that the relative gray values of AQP-1 protein in groups A and C were 0.65±0.06, 0.88±0.11, respectively. The relative gray values of the mRNA expression in groups A and C were 0.30±0.08, 0.49±0.11, respectively. The expression of AQP-1 protein and mRNA in group C was significantly higher than in group A （P 〈0.05）. Conclusion The pretreatment with ginaton can reduce the acute lung injury caused by ischemia/reperfusion.

关 键 词：ischemia-reperfusion injury  aquaporin GINATON PRETREATMENT 

分 类 号：R285[医药卫生—中药学]