尿样中3-硝基酪氨酸在轨检测技术的建立与空间验证  

Establishment and Space Vertification of the On-orbit Monitoring Technology for 3-nitrotyrosine in Urine Samples

作　　者：王春艳[1] 谭映军[1] 顾寅[1] 万玉民[1] 毕蕾[1] 曲丽娜[1] 卫毅[1] 姚宇华[1] 聂捷琳[1] 于建茹[1] 李莹辉[1] 

机构地区：[1]中国航天员科研训练中心,航天医学基础与应用国家重点实验室,北京100094

出　　处：《航天医学与医学工程》2013年第6期463-466,共4页Space Medicine & Medical Engineering

基　　金：中国载人航天工程基金资助项目;航天医学基础与应用国家重点实验室自主资助课题(SMFA11A02)

摘　　要：目的建立尿液中3-硝基酪氨酸(3-nitrotyrosine,3-NT)在轨检测装置和技术,为评估航天员飞行中应激状态变化提供客观依据。方法基于表面等离子体共振(SPR)原理,研制了在轨医监生化检测装置,并建立了配套的尿液预处理及3-NT检测技术,于"神舟"9号任务中进行了在轨飞行验证。结果在轨检测装置空间工作正常,3-NT检测灵敏度好、准确度高、特异性好,圆满完成飞行中尿液测试。结论建立了尿样中3-NT在轨检测技术,成功应用于空间环境。Objective To establish an on-orbit monitoring technology for 3-nitrotyrosine （3-NT） in urine sam- pies, so as to provide objective data for the assessment of the stress status of astronauts during the spaceflight. Methods A Space biochemical monitoring device （SBMD） and the corresponding 3-NT detection technology based on the surface plasmon resonance （SPR） principle were developed, and then tested and verified in SZ-9 manned space mission. Results The device worked well in space and the method showed good sensitivity, ac- curacy, and specificity. The whole system completed the detection successfully. Conclusion An on-orbit mo- nitoring technology for 3-NT in urine samples is established and applied in space firstly.

关 键 词：尿液 3-硝基酪氨酸 表面等离子体共振 在轨监测技术 空间飞行 

分 类 号：R856[医药卫生—航空、航天与航海医学] R854[医药卫生—临床医学]