蓖麻愈伤组织对铜的抗性研究  

Study on Copper-Resistance of the Callus of Ricinus Communis L.

作　　者：康薇[1] 董化洋 金裕华[1] 闵建华[1] 郑进[1,2] 

机构地区：[1]湖北理工学院,湖北黄石435003 [2]矿区环境污染控制与修复湖北省重点实验室,湖北黄石435003

出　　处：《化学与生物工程》2014年第1期26-28,39,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金面上项目(41371470);湖北省自然科学基金重点资助项目(2011CDA037);湖北理工学院大学生科技创新项目(2012cx23)

摘　　要：为了探讨蓖麻愈伤组织对铜的抗性,研究了铜胁迫下蓖麻愈伤组织的增长及其铜吸收作用。结果显示,铜浓度为60mg·L-1时,愈伤组织的增长受到抑制,抗性指数仅为33.87%;铜浓度为40mg·L-1时,愈伤组织呈淡黄色,生长较快,抗性指数达到61.29%,并且这种抗性可以保持至连续继代培养6周之后。培养至第4周,铜浓度(mg·L-1)为10、20、30、40各处理的抗性指数都高于第3周,各处理愈伤组织铜含量(mg·g-1)依次为0.33、0.54、1.16、1.40。培养基铜浓度为40mg·L-1可作为筛选铜抗性蓖麻愈伤组织的临界值。In this paper,the growth of the callus of R. communis L. and its absorption to copper are studied for researching its copper-resistance under different copper concentrations. The results show that the growth of the callus is inhibited nearly under 60 mg ·L^-1copper and its copper-resistance is only 33.87%. The callus can grow rapidly and appear pale yellow with 61.29% of copper-resistance under 40 mg ·L^-1 copper,and the cop- per-resistance even can last for six weeks. After culturing 4 weeks,the copper-resistance are all over those for culturing 3 weeks under 10 mg ·L^-1 copper, 20 mg·L^-1 copper,30 mg ·L^-1 copper,40 mg·L^-1 copper,and the copper concentration are 0.33 mg· g^-1,0.54 mg · g^-1,1.16 mg· g^-1and 1.40 mg · g^-1 in turn. So,it is suggested that the medium containing 40 mg · L^-1 copper is the most appropriate for selecting copper-resistant callus of R. communis L.

关 键 词：蓖麻 铜抗性愈伤组织 筛选 

分 类 号：Q948[生物学—植物学] S792.119[农业科学—林木遗传育种]