HPLC法测定小鼠脑内卡马西平的浓度  

Determination of the drug level of Carbamazepine in mice brain by HPLC

作　　者：冯淑玲[1,2] 王凌[1,2] 陈宇星[1,2] 

机构地区：[1]福建医科大学省立临床学院 [2]福建省立医院药学部,福州350001

出　　处：《海峡药学》2013年第12期186-188,共3页Strait Pharmaceutical Journal

摘　　要：目的 利用高效液相色谱法建立脑内卡马西平浓度的测定方法.方法 脑组织加生理盐水,研磨至匀浆液,离心后取上清液加入乙腈沉淀蛋白.色谱柱为Inertsil ODS-SP柱（4.6×250mm,5μm）,流动相：水∶乙腈（60：40,V/V）,流速1.0mL·min-1,柱温30℃,检测波长235nm.结果 卡马西平线性范围为0.20～20.00μg·g-1（r=0.999）,相对回收率在98.83～102.12%,日内和日间精密度均小于6.9%.结论 该方法便捷、灵敏、准确,可用于卡马西平耐药机理、疗效提高等方面的研究.OBJECTIVE To develop an HPLC method for the determination of carbamazepine in brain. METHODS Brain tissue plus saline was ground to homogenate, and then centrifuged. The supernatant was precipitated of protein by acetonitrile. Chromatography was performed on the Inertsil ODS-SP column （4.6 × 250mm, 5μm）. The mobile phase consisted of a mixture of water-acetonitrile （60：40,v/v） at the flow rate of 1.0mL ·min-1. The column temperature was 30℃ and UV detection wavelength was 235nm. RESULTS The calibration curve was linear over the concentration range of 0. 20 - 20. 00μg · g - 1 （ r = 0. 999 ）. The method recovery was in the range of 98.83 - 102. 12%. The intra-day and inter-day variability values were less than 6. 9%. CONCLUSION The established method is convenient, sensitive, accurate and applicable for the study of resistant mechanism, improvement of the efficacy and so on.

关 键 词：卡马西平 脑 高效液相色谱 测定 

分 类 号：R969.4[医药卫生—药理学]