响应面法优化钙离子沉淀分离田基黄槲皮素  

Optimization on Calcium Ion Precipitation Separation Technology of Quercetin for Hypericum japonicum by Response Surface Methodology

作　　者：欧阳玉祝[1] 魏燕[1] 李雪峰[1] 徐勇威[1] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000

出　　处：《林产化学与工业》2013年第6期116-120,共5页Chemistry and Industry of Forest Products

基　　金：湖南省科技厅科技计划项目(2008FJ3077)

摘　　要：利用响应面(RSM)优化法研究了钙离子沉淀分离田基黄槲皮素的工艺条件。考察了石灰水用量、沉淀温度和沉淀时间对槲皮素得率的影响。结果表明,用64 mL饱和澄清石灰水做沉淀剂,61℃沉淀31 min,槲皮素的得率为3.256%。分离产物用高效液相色谱结合标样进行定性分析,结果与标准品一致。Calcium ion precipitation separation conditions of quercetin from Hypericum japonicum Thumb.were optimized by response surface methodology （RSM）.Influence of calcium hydroxide water solution dosage,precipitation temperature and.times on extraction ratio of quercetin were studied.The resuhs show that extraction rate of quercetin from Hypericum japonicum is 3.256％ with 64 mL calcium hydroxide water solution as precipitator,61 ℃ precipitation for 31 min.The separation product agrees with standard substance by high performance liquid chromatography （HPLC） analysis.

关 键 词：田基黄 槲皮素 离子沉淀 响应面法 

分 类 号：TQ35[化学工程] TQ28