壳聚糖支架与BGC-823细胞的体外生物相容性研究  

Biocompatibility between BGC-823 cells and chitosan scaffold in vitro

作　　者：赵建忠[1] 张广程[1] 狄东华[1] 张庆[1] 

机构地区：[1]江苏大学附属医院骨科,江苏镇江212000

出　　处：《临床检验杂志》2013年第11期856-858,共3页Chinese Journal of Clinical Laboratory Science

基　　金：镇江市社会发展基金资助项目(FZ2012033)

摘　　要：目的探讨壳聚糖（chitosanscaffold，CS）支架与细胞的生物相容性，为CS的体内安全应用提供实验依据。方法采用细胞形态学和MTY法评价CS支架对人胃腺癌细胞系BGC-823生物学特性的影响。在细胞培养液中分别加入不同浓度的明胶固化CS支架浸提液，倒置显微镜下观察各组细胞形态学变化，计数并绘制生长曲线；用MTT法检测并计算细胞相对增殖率。结果不同浓度的CS支架浸提液对体外培养的各组细胞形态无明显影响，对细胞增殖抑制不明显（P均〉0．05），不同浓度CS支架浸提液的细胞毒性均为0～1级。结论CS支架与BGC-823细胞有良好的生物相容性，有望成为理想的医用生物材料。Objective To investgate the biocompatibility between chitosan scaffold （CS） and cells in vitro to provide data for the appli- cation of CSin vivo. Methods The effects of CS for the biological characteristics of human gastric adenocarcinoma cell line BGC-823 were evaluated with cell morphological examination and MTF assay. The cells were co-cultured in the leaching solution containing gelat- in-immobilized CS with a series of concentrations. The morphological changes of the cells were observed under invert microscope per 24-hour and the cell counting was conducted to plot the growth curve. The effects of CS on the relative proliferation rate of BGC-823 ceils were determined by MTT assay. Results No remarkable morphological change in the cells was found during the culture with CS solution in vitro. The leaching solutions of CS showed no significant inhibition on either growth or proliferation of the cells （P 〉 0.05 ）. The cytotoxicity gradation of various concentrations of the leaching solution of CS was ranged from 0 to1 degree. Conclusion CS showed high biocompatibility with BGC-823 cells and may become valuable medical biomaterials.

关 键 词：壳聚糖支架 生物相容性 细胞培养 

分 类 号：R318.08[医药卫生—生物医学工程]