海湾战争综合征模型大鼠海马脑源性神经营养因子及其受体和调节因子的表达  

EXPRESSION OF HIPPOCAMPAL BRAIN-DERIVED NEUROTROPHIC FACTOR AND ITS RELATED RECEPTOR,REGULATORY FACTOR IN A RAT MODEL OF GULF WAR SYNDORME

作　　者：吕彦[1] 何凡[1] 曲方[1] 王而强[1] 张景华[1] 

机构地区：[1]沈阳军区总医院神经内科,沈阳110840

出　　处：《解放军预防医学杂志》2013年第6期490-492,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：沈阳军区总医院重点科研基金课题(No.06Y-Z15);辽宁省科技攻关项目计划资助课题(No.2011225021)

摘　　要：目的探讨脑源性神经营养因子(BDNF)对海湾战争综合征(GWS)海马细胞凋亡或存活的作用及其机制,为应用BDNF预防和/或治疗GWS提供理论依据。方法入选大鼠随机分为对照组、束缚应激组和GWS模型组。实验28 d结束后处死动物,取脑。采用免疫组化技术和免疫印迹分析检测海马CA1区BDNF及其受体酪氨酸蛋白激酶B(TrkB)和cAMP反应元件结合蛋白(CREB)的表达。结果束缚应激组大鼠海马BDNF、TrkB及CREB表达的平均吸光度值分别为0.291±0.001、0.253±0.002、0.215±0.013,明显低于对照组(BDNF 0.320±0.040、TrkB 0.243±0.004、CREB 0.232±0.009)(P<0.05);GWS模型大鼠海马BDNF、TrkB及CREB表达的平均吸光度值分别为0.269±0.036、0.226±0.004、0.194±0.010,明显低于低于对照组和束缚应激组(P<0.05)。结论 GWS模型大鼠海马CA1区BDNF及其受体TrkB以及调节因子CREB的表达明显下降。Objective To explore the role of hippocampal brain-derived neurotrophic factor（BDNF） in hippocampal cell apoptosis or survival in the Gulf War Syndrome （GWS） , so ao to provide theoretical evidence for using BDNF to prevent and/or treat GWS. Methods The rats were randomly divided into three groups： control group, restraint-stress group, and GWS group （established according to Abdel-Rahman et al）. The animals were killed at the end of 28 d-experiment, and the brains were removed to detect the expression of the BDNF, tylrosine related receptor kinase B （TrkB） and cAMP response element binding protein （CREB） in the hippocampal CAI re- gion by using immunohistoehemical staining and Western blot analysis. Results The average absorbance of hipp- ocampal BDNF,TrkB ,and CREB in restraint-stress group （0.291 _±0. 001, 0. 253_±0.002, and 0.215_±0. 013 re- spectively） was significantly lower than those in control group （0.320±0. 040, 0. 243±0.004, 0. 232±0.009 ）, （P〈0.05） ;the average absorbance of hippoeampal BDNF,TrkB, and CREB in GWS group （0.269±0. 036,0.226±0. 004, and 0. 194_±0. 010 respectively） was significantly lower than those in control group and restraint-stress group （ P〈0.05 ）. Conclusion The expression of brain-derived neurotrophic factor, and its receptor（ TrkB）, as well as regulatory factor（CREB） in the hippocampal CA1 region of GWS rat model was significantly decreased.

关 键 词：海湾战争综合征 海马 脑源性神经营养因子 酪氨酸蛋白激酶B CAMP反应元件结合蛋白 

分 类 号：R827.1[医药卫生—临床医学] Q786[生物学—分子生物学]