极端低温胁迫野扇花叶片耐寒生理生化特性的研究  

Physiological and Biochemical Characteristics of the Leaf of Sarcococca ruscifolia Under Extreme Low Temperature Stress

作　　者：王榆枫 

机构地区：[1]遵义市第四中学,贵州遵义563000

出　　处：《贵州林业科技》2013年第4期43-46,共4页Guizhou Forestry Science and Technology

摘　　要：为研究野扇花对低温环境的适应特性,实验对野生野扇花不同休眠时期叶片进行极端低温(18℃、0℃、-5℃、-10℃、-15℃)胁迫处理,测试野扇花游离脯氨酸含量、POD酶活性及细胞膜透性等耐寒能力的抗性指标,研究结果表明:三个不同越冬时期游离脯氨酸含量变化规律基本一致,当温度在-5℃^-10℃时含量较高,说明该温度下叶片脯氨酸具有较强的抗寒能力;POD酶活性测定结果表明野扇花在不同休眠期表现出不同的酶活性,且休眠末期活性最强,深休眠期-5℃时具有较好的抵御低温胁迫的能力,随着胁迫强度加深,酶活性减小;在-10℃^-15℃时,其相对电导率值急剧增大,经非线性回归分析、Logistic曲线拟合求出拐点温度(LT50)为-12.73℃。研究结果表明野扇花越冬期具有相应的耐寒能力。In order to understand the adaptability of Sarcococca ruscifolia to the low temperature environment, the leaves of Sarcocoeca ruscifolia at different dormant periods were treated with different levels of low temperatures （ 18℃, 0 ℃, - 5 ℃, - 10 ℃, - 15 ℃ ） , after which the stress resistance indexes were measured including the free proline content, the POD enzyme activity and the cell membrane permeability. The result showed that the chan- ges of free proline content at three different overwintering period were basically the same, with higher value at - 5 ℃ - - 10 ℃ showing that the plants have a stronger cold resistance at that temperature ; POD assay showed different enzymatic activities at different dormant periods with the strongest activity at the late stage , and the plant showed better ability to resist low temperature stress in deep dormancy at -5 ℃ with decreasing enzymatic activity when the stress intensity deepened; at the temperature of - 10 ℃ - 15 ℃, the relative conductivity increased rapidly, and the inflection point temperature （ LT50 ） was - 12. 73 ℃, which was come out by method of nonlinear regression a- nalysis and logistic curve fitting. The results showed that Sareococca ruscifolia has the corresponding ability to resist cold temperature during overwintering period.

关 键 词：野扇花 低温胁迫 生理生化 抗寒性 

分 类 号：S688[农业科学—观赏园艺]