一清胶囊中指标成分的含量测定  

Determination of index components in Yiqing Capsule

作　　者：蒲晓辉[1] 杨浩[1] 袁琦[1] 孙立君[1] 杨雪芳[1] 刘绪强[1] 杨文亮[1] 杨颜滋[1] 

机构地区：[1]河南大学药学院药物分析教研室,河南开封475004

出　　处：《河南大学学报（医学版）》2013年第4期247-249,共3页Journal of Henan University:Medical Science

基　　金：国家级大学生创新创业训练计划项目(201210475062);河南大学自然科学基础研究项目(2010YB2R019);河南大学2012年度大学生创新性实验计划项目(12NA013)

摘　　要：目的建立同时分离测定一清胶囊中大黄酸和大黄素含量的反相高效液相色谱法。方法采用Kromasil ODS-C18色谱柱（200mm×4.6mm,5.0μm）,流动相A为0.003 3%三乙胺甲醇溶液（冰醋酸调pH=3.00）,流动相B为体积分数为0.5%三乙胺水溶液（冰醋酸调pH=3.00）,以A∶B=85∶15进行等度洗脱,检测波长430nm,流速1.0mL·min-1。结果一清胶囊中大黄酸和大黄素含量测定方法的线性关系良好,相关系数（r）分别为0.999 6、0.999 7;回收率分别为97.27%~102.0%。结论该法专属性强,结果准确可靠,重复性好,可用于一清胶囊中大黄酸和大黄素的含量测定,更好地控制一清胶囊的质量。Objective To build a RP-HPLC method of determining simultaneously rheinic acid and archen in Yiqing Capsule with gradient elution. Methods A column of Kromasil C18 （ 200 mm× 4.6 mm, 5 μm） was used. The mobile phase was composed of 0. 0033% triethylamine methanol solution （tuning pH to 3.0 with glacial acetic acid, A）, 0.5% triethylamine （tuning pH to 3.0 with glacial acetic acid, B）. Isocratic elution was carried out with A ： B=85 ： 15. The flow rate was 1.0 mL.min 1 with the detection wavelength at 430 nm. Results the ranges, the linears of five rhubarb anthraquinones were very good and the correlation coefficients were 0. 999 6 and 0. 999 7. The average recoveries were 100.1% and 101.4. Conclusion The method had strong specificity. The results were accurate and reliable, repeatable. It can be used to determining rheinic acid and archen, and preferably control the quality of Yiqing Capsule.

关 键 词：反相高效液相法 一清胶囊 大黄酸 大黄素 

分 类 号：R917[医药卫生—药物分析学]