低温对人脐血内皮祖细胞的影响  

The effection of hypothermia to human umbilical cord blood endothelial progenitor cells

作　　者：张蕾[1] 钟晓鸣[1] 程冠昌[1] 洪岩[1] 万琪琳[1] 

机构地区：[1]河南大学淮河医院,河南开封475000

出　　处：《河南大学学报（医学版）》2013年第4期276-279,共4页Journal of Henan University:Medical Science

基　　金：河南大学校内科研基金(2010YBZR036)

摘　　要：目的将人脐血中分离培养出的内皮祖细胞进行低温保存和快速复苏,建立EPC细胞系,比较冻存前后细胞形态及生物学功能是否改变。方法采集人脐血,分离出单核细胞,对细胞进行冻存和复苏,测定内皮祖细胞增殖能力及黏附能力。结果内皮祖细胞经冻存和复苏后测得细胞的存活率为(84.17±4.02)%,细胞的形态学未见明显改变;与未冻存的细胞相比,刚复苏后即行增殖和黏附能力的检测,细胞的增殖和黏附能力有显著变化,P<0.05;细胞复苏后继续培养48h行增殖和黏附能力检测,两者未见明显差异,P>0.05。结论对低温保存后复苏的EPC,立即行增殖和黏附能力检测其能力均降低,复苏后继续培养48h再次检测未见明显差异。Objective Isolating and culturing human umbilical cord blood progenitor cells , then the cells were preserved in the low temperature and then were recoveried quickly. We get the EPC cells, and then compare cryopreservation of cell morphology and biological function. Methods Collection of human umbilical cord blood and isolation and culture of the mononuclear cells. Then we cryopreservation and recovery of cells and measurement of the proliferation activity for EPC. Results Cryopreservation and recovery of the cells： After cryopreservation and recovery, survival rate of EPC which was cultured in vitro was （84.17±4.02）% and there was no significant change in cell morphology. Changes of cell proliferative activity and adherence ability： Compared with non-cryopreserved cells, the cryopreserved cells showed a significant change in proliferative activity and adherence ability （ P 〈 0.05）. After incubation for 48h after recovery the cryopreserved cells, there was no significant difference （ P〉 0.05） of proliferative activity and adherence ability between non-cryopreserved cells and cryopreserved cells. Conclusion We detected the proliferation and adhesion capacity of EPC immediately which was recoveried after shortterm cryopreservation. It shows that the capacity is decreased but there was no significant difference for them after recoveried and cultured 48 h.

关 键 词：内皮祖细胞 细胞培养 冻存 复苏 

分 类 号：R331.1[医药卫生—人体生理学]