党参花粉多糖的研究  

Studies on the Polysaccharides from the Pollen of Codonopsis Pilosula (Franch. ) Nannf.

作　　者：刘站柱[1] 朱国芬[1] 金声[1] 

机构地区：[1]北京大学化学系,北京100871

出　　处：《高等学校化学学报》1991年第12期1600-1604,共5页Chemical Journal of Chinese Universities

摘　　要：从蜜峰采集的党参花粉中,分离到5种多糖CPA～CPE,经凝胶过滤及电泳法验证了纯度并测定了分子量。对CPA进行了全水解、甲基化分析、NalO_4氧化和Smith降解分析,用旋光法测定了CPA中单糖残基的绝对构型,对CPA的IR、~1H NMR、^(13)C NMR及~1H NMR、^(13)C偶合NMR进行了分析,确定了异头碳的构型。对CPA进行了电子显微镜分析,首次在透射电子显微镜下观察并拍摄到了具有串珠状特殊二级结构的多糖照片,经分析得到了CPA二级结构的一些信息。Five water-soluble polysaccharides, CPA, CPB, CPC, CPD and CPE,were isolated and purified from the pollen of Codonopsis Pilosula. Their purity was identified by gel-filtration and electrophoresis. The average molecular weights were estimated by gel-filtration. Hydrolysis, methylation, periodate oxidation and Smith degradation methods were used in the structural studies of CPA. The absolute configurations of the monosaccharides of CPA were identified by measuring the optical rotation of the mixture of the monosaccharides. IR, 1H NMR, 13C NMR and 1H , 13C coupled NMR spectra of CPA were analysed. The transmission electron microscopy (TEM) investigation of CPA showed a clear secondary structural picture. Some information was attained about the structural feature through analysing the micrograph of CPA.

关 键 词：党参 花粉 多糖 电子显微镜 

分 类 号：Q949.783.2[生物学—植物学]