双虎清肝颗粒对刀豆球蛋白A所致肝损伤的防护作用机制研究  

Mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury induced by ConA

作　　者：刘添[1] 周建平[2] 李绍旦[3] 

机构地区：[1]解放军总医院肝胆外科,北京硕士研究生100853 [2]军事医学科学院毒物药物研究所 [3]解放军总医院中医科,北京100853

出　　处：《环球中医药》2013年第12期881-884,共4页Global Traditional Chinese Medicine

基　　金：军队中医药科研专项课题(10ZYZ241)

摘　　要：目的从一氧化氮(nitric oxide,NO)及总蛋白巯基(total sulfhydryl,TSH)、蛋白巯基(protein-bound sulfhydryl,PSH)的变化来探讨双虎清肝颗粒对刀豆球蛋白A(Concanavalin A,ConA)所致肝损伤的防护作用机制。方法 84只BALB/c雄性小鼠随机分成三组,正常对照组、模型对照组和双虎清肝颗粒组各28只。正常对照组给予生理盐水0.2 ml尾静脉注射;模型对照组尾静脉注射ConA(15 mg/kg)生理盐水溶液0.2 ml;双虎清肝颗粒组每日给予双虎清肝颗粒(5 mg/kg)生理盐水溶液2 ml灌胃一次,连续7天后再按模型对照组处理。观察3小时、6小时、9小时及12小时时血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)及NO2-含量以及肝组织TSH、PSH含量的变化情况。结果双虎清肝颗粒组各时间点血浆ALT、AST及NO2-的含量与正常对照组比较均无统计学差异(P>0.05),而与模型对照组比较均存在显著性差异(P<0.05)。双虎清肝颗粒组各时间点肝组织TSH、PSH含量与正常对照组比较均无统计学差异(P>0.05),而与模型对照组比较均有显著性差异(P<0.05)。结论双虎清肝颗粒防护肝损伤的作用机制可能是通过抑制机体NO的生物合成从而降低NO含量、阻断肝细胞TSH及PSH被持续性消耗从而提高肝组织细胞的抗氧化应激能力和解毒能力等方式实现的。Objective Investigate mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury induced by ConA through detecting the level of NO（ nitric oxide）,TSH（ total sulfhydryl） and PSH（ protein-bound sulfhydryl）. Methods 84 male BALB / c mice were randomly divided into three groups. NS（ normal saline,0. 2 ml） was injected via the tail vein to be regarded as the normal group. ConA（ 15 mg / kg） in NS（ 0. 2 ml） was injected via the tail vein to be served as the model group. Shuanghu Qingan Granules（ 5 mg / kg） was given in NS（ 2 ml） by intragastric administration everyday in a week before ConA was injected via the tail vein to be served as the Shuanghu Qingan group. Plasma samples for ALT,AST and NO 2^-measurements were obtained at 3,6,9 and 12 hours after respective treatments in each group,meanwhile content of TSH and PSH of 1iver tissue homogenate. Results In the Shuanghu Qingan group,level of ALT,AST and NO 2^-in plasma changed indistinctively at different time,vs the normal group P〈0. 05 and vs the model group P〈0. 05 respectively. Meanwhile content of TSH and PSH in 1iver tissue homogenate of the Shuanghu Qingan group also changed indistinctively at different time,vs the normal group P〈0. 05 and vs the model group P〈0. 05 respectively. Conclusion The mechanism of pro- tective effect of Shuanghu Qingan Granules on hepatic injury is partly due to inhibiting the biosynthesis of NO significantly and preventing the consumption of TSH and PSH markedly.

关 键 词：双虎清肝颗粒 肝损伤 作用机制 一氧化氮 巯基 

分 类 号：R285.5[医药卫生—中药学]