巨瓣兜兰组培快繁技术研究  

Study on Tissue Culture and Rapid Propagation of Paphiopedilum bellatulum （ Rchb. f. ） Stein

作　　者：王莲辉 姜运力 余登利[2] 魏鲁明[2] 冯育才 田凡 

机构地区：[1]贵州省林业科学研究院,贵阳550005 [2]贵州茂兰国家级自然保护区管理局,荔波558400 [3]贵州大沙河省级自然保护区管理局,道真563000

出　　处：《天然产物研究与开发》2013年第B12期86-89,共4页Natural Product Research and Development

基　　金：基金项目：贵州省林业厅年度计划项目（2008-01）;贵州省科技厅“十一·五”农业攻关项目（2010-3029）;贵州省创新能力平台项目（2009-4002）

摘　　要：在巨瓣兜兰的组培快繁过程中，有机添加物10％的椰乳对原球茎的诱导有极显著影响；激素组合6-BA和NAA及配比10：1对芽的诱导和增殖起明显的促进作用；一定浓度的IBA对巨瓣兜兰的壮苗生根起关键性作用因素；栽培基质的选择极大地影响巨瓣兜兰的移栽成活率。In this study,tissue culture and rapid propagation of Paphiopedilum beUatulum with different treatments were conducted. The results showed that：l ） organic amendment of 10% coconut milk significantly affected the induction rate of protoconn;2） hormone combination of 6-BA and NAA with formulation of 10：1 obviously enhanced the induction and proliferation of buds;3 ） IBA at the proper concentration played the key role in the process of rooting and seedling growth ;4） culture medium selection was a critical factor greatly influencing the survival rate of the transplants.

关 键 词：巨瓣兜兰 组培 快繁 

分 类 号：S68[农业科学—观赏园艺]