小鼠载脂蛋白E基因的组织表达谱分析  

ANALYSIS OF TISSUE-SPECIFIC EXPRESSION PROFILE OF MOUSE APOE

作　　者：赖青鸟[1] 畅荣妮[1] 袁广之[2] 马军[1] 杨益金 陆俊佳[3] 侯伟[1] 舒伟[1] 袁志刚[1] 

机构地区：[1]广西医科大学基础医学院细胞生物学与遗传学教研室,南宁530021 [2]广西医科大学第一附属医院创伤骨科手外科,南宁530021 [3]广西医科大学第三附属医院检验科,南宁530031

出　　处：《广西医科大学学报》2013年第6期847-849,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.2010GXNSFA013155)

摘　　要：目的:探讨小鼠载脂蛋白E(apoE)基因的组织表达特点。方法:提取小鼠的肝、脑和心等17个组织的RNA,采用RTPCR的方法对apoE基因在上述组织中的表达进行研究。结果:apoE基因在肝组织表达最强,其次是脊髓和脑,肺、肾、心、脾、胸腺、小肠、胃、膀胱、血液、肾上腺、子宫、胰腺和主动脉也有不同程度的表达,但在腹肌中几乎不表达。结论:小鼠的apoE基因组织表达谱与人的apoE基因组织表达谱有较高的一致性,与猪的相比差异也不大。Objective： To analyze the tissue-specific expression profile of mouse apoE gene. Methods： The RNA was isolated from seventeen tissues of mouse and the method of RT-PCR was performed to study the tissue expression of mouse apoE gene. Results： Compared with the rest of the tissues, the expression level of apoE gene was highest in the liver, secondly in the spinal marrow and brain. There were different ex- pression levels in other tissues such as the lungs, kidneys, heart, spleen, thymus, small intestine, stom- ach, bladders, blood, adrenal glands, uterus, pancreas and aorta, whereas almost no expression in the ab- dominal muscle. Conclusion： The tissue-specific expression in the profile of the mouse apoE gene is similar to those of human and pig.

关 键 词：小鼠 载脂蛋白E 组织表达谱 

分 类 号：Q344.13[生物学—遗传学]