高通量细胞培养芯片的设计及应用(英文)  

High-throughput microwell plate for cell research

作　　者：杜岗[1] 黄正一[1] 常青[2] 李自成[1] 

机构地区：[1]暨南大学附属第一医院心内科,广东广州510630 [2]暨南大学医学院组胚教研室,广东广州510632

出　　处：《南方医科大学学报》2014年第1期5-7,共3页Journal of Southern Medical University

基　　金：Supported by Key Discipline Construction Program of First Affiliated Hospital of Jinan University~~

摘　　要：作者设计了一种简单的、不需要复杂管道和动力泵的细胞培养芯片。通过这种芯片可以精确地观察单个或几个细胞的增殖、凋亡的过程,在自动显微成像的帮助下可以详细记录单个细胞对药物的反应等。这种芯片可以在普通的实验室设计和制作,可以放在小的培养皿中,所以很容易把它放到培养箱,从而使细胞有一个稳定的生长环境。作者以PC-12细胞为例,观察了该细胞在这种培养芯片中的生长、增殖和凋亡的过程,证明了这种设计的可行性。同时,这种细胞培养芯片可以很大程度上节约细胞标本和试剂的应用,比较适合稀少样本或者试剂昂贵的细胞学实验。We developed a novel simple microwell plate that allows researchers to culture cells on chips easily without the use of tubes and pumps for studies of cell proliferation, apoptosis and pharmacology. Unlike any other previous design, this device can be put into an incubator directly and maintains cells in an environment with stable temperature, humidity, air pressure, oxygen and CO2 concentration. We cultured PC-12 cells in these microwell plates and measured the cell proliferation and apoptosis. The microwell plate is recommended for use in all research including those involving rare samples and expensive reagents.

关 键 词：细胞培养芯片 单细胞观察 PC-12细胞 

分 类 号：R318.6[医药卫生—生物医学工程]