低强度超声对早孕大鼠药物流产后子宫组织中内皮素-1和一氧化氮的影响  

Effect of low-intensity ultrasound on endothelin-1 and nitrogen monoxidum in uterine tissues of SD rats following abortion

作　　者：张艳霞[1] 郭菊芳[1] 林川[1] 陆露[1] 李成志[1] 

机构地区：[1]重庆医科大学生物医学工程学院//省部共建超声医学工程国家重点实验室//超声医学工程重庆市重点实验室,重庆400016

出　　处：《南方医科大学学报》2014年第1期100-102,共3页Journal of Southern Medical University

基　　金："十二五"国家科技支撑计划项目(2011BAI141301);重庆市教委科研项目(KJ110325)~~

摘　　要：目的观察低强度超声对早孕大鼠药物流产后子宫组织内皮素-1(ET-1)和一氧化氮(NO)的影响。方法雌性早孕大鼠30只,随机分成治疗组和对照组,采用米非司酮和米索前列醇造成流产模型,治疗组给予低强度超声治疗30 min/d,共5 d,对照组采用与治疗组相同的操作方法进行治疗,但仪器无功率输出。5 d后取子宫组织制成匀浆,分别用酶联免疫法和化学测试法检测ET-1和NO的含量。结果治疗组子宫组织中ET-1含量明显高于对照组(P<0.05);NO含量差异无显著性(P>0.05)。结论低强度超声可能通过增加子宫组织中内皮素-1含量,改变ET-1与NO的动态平衡,从而促进子宫平滑肌的收缩。Objective To investigate the effect of low-intensity ultrasound on the contents of endothelin （ET-1） and nitrogen monoxidum （NO） in uterine tissues of SD rats after abortion. Methods Thirty female pregnant rats were randomly divided into treatment group and control group and received mifepristone and misoprostol to induce abortion. The rats in the treatment group were treated by low-intensity ultrasound for 30 min/day for 5 consecutive days, and those in the control group received sham treatment. The uterine tissue was then taken and homogenized for measurement of ET-1 and NO contents using enzyme-linked immunosorbent assay and chemical testing. Results ET-1 content in the uterine tissues was significantly higher in the treatment group than in the control group （P〈0.05）, but NO content showed no significant difference between the two groups （P〉0.05）. Conclusion Low-intensity ultrasound can promote the contraction of uterine smooth muscles by increasing the level of ET-1 to modulate the homeostasis of ET-1 and NO.

关 键 词：低强度超声 早孕 药物流产 子宫 一氧化氮 内皮素-1 

分 类 号：R445.1[医药卫生—影像医学与核医学]