河南地区猪嵴病毒分子检测和VP1基因遗传进化分析  

Detection and phylogenetic analysis of porcine Kobuvirus VP1 in Henan province

作　　者：姬郭彪[1] 赵军[1] 周峰[1] 魏亚鹏[1] 常洪涛[1] 郑逢梅[1] 王川庆[1] 

机构地区：[1]河南农业大学,河南郑州450002

出　　处：《中国预防兽医学报》2014年第1期73-76,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31072116;31272567);河南省各项青年骨干教师资助计划(2010GGJS-045)

摘　　要：猪嵴病毒（PKV）为新发现的小RNA病毒科嵴病毒属的一个待定种。为调查PKV在河南地区猪群中的感染和流行情况，本研究采集2010年~2012年河南地区84个种猪场150份猪腹泻粪便样品和23份无腹泻症状猪的粪便样品（猪场有腹泻史），采用RT．PCR方法对PKV的VP1基因进行检测。结果显示，检测样品中PKV总阳性率为82．1％（142／173），猪场PKV总阳性率为82．1％（69／84），表明河南地区各种猪群普遍存在PKV感染。其中发病猪群的PKV总阳性率为80．0％（120／150），临床健康猪群的PKV总阳性率为95．7％（22／23），两者无明显差异。此外，对8个阳性样品中VP1基因进行测序比对，结果显示与中国其它地区的PKVvP1基因的核苷酸同源性为80．7％～99．9％，推导的氨基酸序列同源性为83．9％～99．6％。遗传进化分析显示所有已知的PKV株构成4个分支，河南的8个PKV的VP1序列分别位于其中的3个分支中。Porcine Kobuvirus （PKV） is a new candidate species of the genus Kobuvirus in the family Picornavin＇dae. To evaluate the epidemic situation of PKV in pig herds in Henan province, a total of 150 fecal samples from pigs with diarrhea and 23 fecal samples from clinical healthy pigs were collected from 84 pig farms during 2010 to 2012, and PKV in these samples was detected by using RT-PCR. The result revealed that 142 （82.1%） samples and 69 （82.1%） farms were positive for PKV, which indicated that PKV infections in pig herds are endemic in Henan province. The PKV positive rate was 80.0% （120/150） and 95.7% （22/23） in porcine samples collected from pigs with diarrhea and clinical healthy pigs, respectively. VP1 gene sequences of 8 samples positive for PKV were cloned and sequenced for the phylogenetic analysis, the nucleotide identities between the 8 sequences and other reported sequences from different parts of China ranged from 80.7% to 99.9%, and the identities were 83.9% to 99.6% at the amino acid level. The 8 VP1 sequences distributed in three of four branches of the phylogenetic tree constructed by MEGA 4.0.

关 键 词：猪嵴病毒 VP1基因 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]