低硒对小鼠心肌钾离子通道基因mRNA表达的影响．  

Expression of potassium ion channel mRNA in myocardial tissue of mice with low selenium

作　　者：孙美娜[1] 李凤兰[1] 赵汉东[1] 李晖[1] 

机构地区：[1]哈尔滨医科大学基础医学院生物化学与分子生物学教研室,150081

出　　处：《中华地方病学杂志》2014年第1期46-48,共3页Chinese Journal of Endemiology

基　　金：国家自然科学基金（30771863、81172616）

摘　　要：目的研究钾离子通道基因mRNA在低硒小鼠心肌组织中的表达。方法C57BL／6小鼠40只，雌雄各半，体质量18～22g，按体质量随机分为4组，每组10只小鼠（雌雄各半），其中低硒4周组、12周组、24周组分别采用低硒饲料（硒含量为0．004mg／kg）喂养4周、12周、24周，对照组则给予正常饲料（硒含量为0．256mg／kg）。将各组小鼠断颈处死，取小鼠心脏，Trizol法提取RNA，采用实时荧光定量PCR（reM—timePCR）方法检测钾离子通道基因（KCNA4、KCND2、KCND3、KCNEl、KCNE2、KCNJ2、KCNJl2、KCNQl）mRNA的表达。结果低硒4周组钾离子通道基因KCNA4、KCND2mRNA[（25．3±0．09）、（4．85±0．05）]表达高于对照组（1．00±0．00、1．00±0．00，P均〈0．05）；低硒24周组钾离子通道基因KCNJl2mRNA（22．7±0．10）表达高于对照组（P〈0．05）；KCND3、KCNEl、KCNE2、KCNJ2、KCNQlmRNA表达与对照组比较差异无统计学意义（P均〉0．05）。结论低硒能够影响小鼠心肌钾离子通道基因的表达。Objective To examine the expression of potassium channel mRNA in myocardial tissue of mice with low selenium. Methods Forty C57BL/6 mice were randomly divided into four groups according to body weight（18 - 22 g）, 10 mice in each group, half male and half female. The low-selenium treatment groups were fed with low-selenium diet（selenium content was 0.004 mg/kg） for 4, 12 and 24 weeks, respectively, and the control group was fed with normal diet（selenium content was 0.256 mg/kg）. The mice were killed by cutting neck method, hearts were taken out and RNA was extracted by Trizol method. The expressions of potassium ion channel genes （KCNA4, KCND2, KCND3, KCNE1, KCNE2, KCNJ2, KCNJ12 and KCNQ1） at the mRNA level in heart were determined using real-time polymerase chain reaction. Results In low-selenium 4 weeks group, the mRNA expressions of KCNA4 gene（25.3 ± 0.09） and KCND2 gene（4.85 ± 0.05） were higher than that of the control group （1.00 ± 0.00, 1.00 ± 0.00, all P〈 0.05）; in low-selenium 24 weeks group, the mRNA expression of KCNJ12 gene （22.7 ± 0.10） was higher than that of the control group （1.00 ± 0.00, P 〈 0.05）; the mRNA expressions of KCND3, KCNE1, KCNE2, KCNJ2, KCNQ1 genes were compared with the corresponding control groups, the differences were not statistically significant（all P 〉 0.05）. Conclusions Low selenium can affect the mRNA expression of mouse cardiac potassium ion channel genes. [

关 键 词：硒 小鼠 钾离子通道 

分 类 号：R54[医药卫生—心血管疾病]